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Packaging of RVFV pseudoviruses and establishment of a neutralization assay method
Yuetao Li1,2,3,†, Yongkun Zhao2,†, Cuiling Wang1,2, Xuexing Zheng2,4, Hualei Wang2, Weiwei Gai2,5, Hongli Jin6, Feihu Yan2, Boning Qiu2,5, Yuwei Gao2, Nan Li2, Songtao Yang2, Xianzhu Xia1,2,*
1College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, China
2Institute of Military Veterinary Medicine, Academy of Military Medical Science, Changchun, China
3College of Animal Science and Technology, Shihezi University, Shihezi, China
4School of Pubulic Health, Shandong University, Jinan, China
5College of Veterinary Medicine, Jilin University, Changchun, China
6Changchun SR Biological Technology Co., Ltd., Changchun, China
Correspondence to: Tel: +86-431-8698-5928; Fax: +86-431-8698-5869; E-mail: xiaxzh@cae.cn
The first two authors contributed equally to this work.
Received: December 19, 2016; Revised: March 1, 2017; Accepted: May 5, 2017; Published online: July 10, 2017.
Abstract
Rift Valley fever (RVF) is an acute, febrile zoonotic disease that is caused by the RVF virus (RVFV). RVF is mainly prevalent on the Arabian Peninsula, the African continent, and several islands in the Indian Ocean near southeast Africa. RVFV has been classified by the World Organisation for Animal Health (OIE) as a category A pathogen. To avoid the biological safety concerns associated with use of the pathogen in RVFV neutralization assays, the present study explored and established an RVFV pseudovirus-based neutralization assay. This study specifically used the HIV lentiviral packaging system and RVFV structural proteins to successfully construct RVFV pseudoviruses. Electron microscopy observation and western blotting indicated that the size, structure, and shape of the packaged pseudoviruses were highly similar to those of HIV lentiviral vectors. Infection inhibition assay results showed that an antibody against RVFV inhibited the infective ability of the RVFV pseudoviruses, and an antibody neutralization assay for RVFV detection was then established. Therefore, this study successfully established a neutralization assay based on RVFV pseudoviruses and demonstrated that this method can be used to effectively evaluate antibody neutralization.
Keywords: Rift Valley fever virus, antibody neutralization assay, pseudovirus


© 2017 The Korean Society of Veterinary Science.