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Comparison the characteristics of canine adipose tissue-derived mesenchymal stem cells extracted from different sites and at different passage numbers
Kevin M. Yaneselli1, Cristiana Palma Kuhl2, Paula Barros Terraciano2, Fernanda de los Santos de Oliveira2, Sabrina Beal Pizzato2, Kamila Pazza2, Alessandra Bileski Magrisso2, Vanessa Torman3, Analía Rial4, María Moreno4, Silvia Llambí5, Elizabeth Cirne Lima2, Jacqueline Maisonnave1,*
1Laboratory of Immunology, Department of Microbiological Science, Facultad de Veterinaria, Universidad de la República, Lasplaces 1550, Montevideo 11600, Uruguay
2Laboratory of Embryology and Cellular Differentiation, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS 90035-903, Brazil
3Biostatistics, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS 90035-903, Brazil
4Laboratory for Vaccine Research, Department of Biotechnology, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Montevideo 11600, Uruguay
5Laboratory of Genetics, Facultad de Veterinaria, Universidad de la República, Montevideo 11600, Uruguay
Correspondence to: Tel: +598-26281303; Fax: +598-26280130; E-mail: jacmaiso@gmail.com.
Received: December 28, 2016; Revised: April 25, 2017; Accepted: May 5, 2017; Published online: July 10, 2017.
Abstract
Mesenchymal Stem Cells (MSCs) have attractive characteristics for therapy in animal models and veterinary medicine, due to the capacity of inducing tissue regeneration and immunomodulation. The objective of this study was to evaluate the differences between canine adipose tissue-derived MSCs (AD-MSCs) extracted from subcutaneous (Sc) and visceral (Vs) sites. Surface antigenic markers, in vitro differentiation and mineralized matrix quantification of AD-MSCs in different passages (P4, P6 and P8) were studied. Immunophentoypic analysis showed that AD-MSCs from both sites were CD44+ CD90+ and CD45-. Moreover, they were able to, in vitro differentiate into, fat, cartilage and bone. Sc AD-MSCs preserve its in vitro multipotentiality up to P8, but Vs AD-MSCs only tridifferentiated up to P4. In addition, Sc cells had greater capacity of in vitro mineralized matrix synthesis. In conclusion Sc derived cells have advantages over Vs, as Sc AD-MSCs preserve multipotentiality during more passages, have a more osteogenic potential and its extraction is less invasive.
Keywords: canine, immunophenotyping, different extraction sites, mesenchymal stem cells, multipotent plasticity


© 2017 The Korean Society of Veterinary Science.