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Tannic acid-mediated immune activation attenuates Brucella abortus infection in mice
Alisha Wehdnesday Bernardo Reyes1,2, Huynh Tan Hop1, Lauren Togonon Arayan1, Tran Xuan Ngoc Huy1, Wongi Min1, Hu Jang Lee1, Hong Hee Chang3, Suk Kim1, 3,*
1Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang and 3Institute of Agriculture and Life Science, National University, Jinju 52828, Korea
2Department of Veterinary Paraclinical Sciences, College of Veterinary Medicine, University of the Philippines Los Baños, College, Laguna 4031, Philippines
Correspondence to: Tel: +82–55–772–2359; Fax: +82–55–772–2349; E-mail: kimsuk@gnu.ac.kr,
Received: January 9, 2017; Revised: March 17, 2017; Accepted: May 5, 2017; Published online: July 10, 2017.
Abstract
Brucellosis is an emerging infectious disease affecting humans and animals. In this study, we investigated the in vitro and in vivo effects of tannic acid (TA) against Brucella (B.) abortus infection. Reduced F-actin polymerization and MAPKs (ERK 1/2 and p38α) phosphorylation were observed in TA-treated cells as compared with control cells after infection. The mice were infected via intraperitoneal route and were orally given with TA or PBS for 14 days. The weights of the spleens from TA-treated and control mice were not different, however the splenic proliferation of B. abortus was significantly reduced in treated group. For the analysis of immune response, the non-infected TA-treated mice displayed increased levels of IFN-γ, MCP-1 and IL-10 at 3 days post-infection, with consistent increase in IFN-γ and MCP-1 at 14 days post-infection as compared with the control group. On the other hand, the infected TA-treated mice displayed elevated levels of IFN-γ at 3 days post-infection which continuously increased at 14 days post-infection with the addition of TNF as compared with the control group. Taken together, these findings highlight the potential influence of TA in the control of Brucella infection via activation of cytokine production and inhibition of bacterial proliferation in the host.
Keywords: Brucella abortus, F-actin, cytokines, mitogen-activated protein kinase, tannic acid


© 2017 The Korean Society of Veterinary Science.