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Polymorphisms in the serotonin transporter protein gene in Maltese dogs with degenerative mitral valve disease
Chang-Min Lee1, Jae-Ik Han2, Min-Hee Kang1, Seung-Gon Kim1, Hee-Myung Park1,*
1Department of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University, Seoul 05030, Korea
2Laboratory of Wildlife Diseases, College of Veterinary Medicine, Chonbuk National University, Iksan 54596, Korea
Correspondence to: Tel: +82-2-450-4140; Fax: +82-2-450-4140; E-mail: parkhee@konkuk.ac.kr
Received: January 11, 2017; Revised: April 29, 2017; Accepted: June 21, 2017; Published online: July 10, 2017.
Degenerative mitral valve disease (DMVD) is the most commonly acquired cardiac disease in dogs. This study was conducted to evaluate the relationship between genetic variations in serotonin transporter (SERT) gene of Maltese dogs and DMVD. Twenty client-owned DMVD Maltese dogs and 10 healthy control dogs. Genomic DNA was extracted from blood samples collected from these dogs, and each exon of the SERT gene was amplified using polymerase chain reaction. The resulting genetic sequences were aligned and analyzed for variations by using the reference sequences; the predicted secondary structures of these variations were modeled and cross-verified by computational methods. Genetic variations were detected in five exons, including five nonsynonymous genetic variations. The protein structure and function of the five nonsynonymous genetic variations were predicted. Three of the five polymorphisms were predicted as probable causes of damage to protein function and confirmed by protein structure model verification. This study identified six polymorphisms of the SERT gene in Maltese dogs with DMVD, it suggested as an evidence of association between the SERT gene and canine DMVD. This is the first study of SERT mutation in Maltese dogs with DMVD and this is a pilot study of clinical genetic examination for early diagnosis.
Keywords: canine, mitral valve, polymorphism, serotonin transporter

© 2017 The Korean Society of Veterinary Science.