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Isolation and characterization of a new porcine epidemic diarrhea virus variant that occurred in Korea in 2014
Dong-Kun Yang*, Ha-Hyun Kim, Seong-Hun Lee, Soon-Seek Yoon, Joong-won Park, In-Soo Cho
Animal and Plant Quarantine Agency, MAFRA, Gimcheon, 39660, Korea
Correspondence to: Tel: +82-54-912-0785; Fax: +82-54-912-0812; E-mail: yangdk@korea.kr
Received: January 13, 2017; Revised: May 24, 2017; Accepted: June 29, 2017; Published online: July 10, 2017.
Abstract
Porcine epidemic diarrhea (PED) has caused significant economic losses in the swine industry, and another epidemic occurred recently in Korea in 2014. Isolating and culturing the PED virus (PEDV) allow investigations into its pathogenesis and development of vaccines and diagnostic assays. In this study, we successfully isolated two PEDV isolates (QIAP1401 and QIAP1402) from naturally infected piglets in Jeju-do. Viral propagation was confirmed in Vero cells according to cytopathic effects, immunofluorescence assay, reverse transcription-polymerase chain reaction and electron microscopic analyses. The QIAP401 isolate propagated well in Vero cells for 70 passages, with titers of 106.5 to 107.0 50% tissue culture infectious dose/mL, which increased gradually with passaging. The nucleotide and amino acid sequences of the QIAP1401 isolate were determined and compared with those of other PEDV isolates. The QIAP1401 isolate was determined to be closely related to the USA/Minnesota271/2014 strain (>99.9% nucleotide similarity), which was isolated in the U.S. in 2014. A phylogenetic analysis based on several PEDV genes suggested that a new PEDV variant is circulating in the Korean swine industry, with 93.08% similarity to the SM98 strain isolated in 1998. In addition, the QIAP1401 strain showed strong virulence in 3-day-old piglets and 11-week-old growing pigs.
Keywords: isolation, porcine epidemic diarrhea virus, swine


© 2017 The Korean Society of Veterinary Science.