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Tibial dyschondroplasia is highly related to suppression of the hypoxia inducible factor (HIF)-1ɑ,-2ɑ, and -3ɑ expressions in chickens
Shucheng Huang1, Mujeeb Ur Rehman1, Gang Qiu1,2, Houqiang Luo1,3, Muhammad Kashif Iqbal1, Hui Zhang1, Khalid Mehmood1, Jiakui Li1,2,*
1College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China
2Laboratory of Detection and Monitoring of Highland Animal Disease, Tibet Agriculture and Animal Husbandry College, Linzhi 860000 Tibet, China
3Animal Science Department, Wenzhou Vocational College of Science and Technology, Wenzhou 325006, China
Correspondence to: Tel: +86-27-87286251; E-mail: lijk210@sina.com
Received: February 7, 2017; Revised: April 4, 2017; Accepted: May 5, 2017; Published online: July 10, 2017.
Abstract
Tibetan chickens (TBCs) live at high altitude hypoxic environment. None of the Tibial dyschondroplasia (TD) cases had been reported in TBCs, which is commonly seen in commercial broilers characterized by lameness. The underlying mechanism remains unclear. Hypoxia inducible factors (HIFs) are important regulators of cellular adaptation to hypoxic conditions. Here we investigated the role of HIF-1ɑ, -2ɑ, and -3ɑ in hypoxia and thiram-induced TD and their effect on tibial growth plate development in Acres chickens (AACs) and TBCs. RNA and protein expression levels of HIF-1ɑ, -2ɑ, and -3ɑ were tested using qRT-PCR and western blotting analysis, respectively. Interestingly, the results showed that HIF-1ɑ, -2ɑ, and -3ɑ expressions in the tibial growth plate of TBCs were up-regulated by hypoxia and were more significant than AACs. However, these factors were down-regulated in thiram-induced TD experiment. To further clarify the effect of thirm on tibial growth plate of commercial broilers, Arbor Acres chickens (AACs) were observed with more pronounced changes in their growth plate. Altogether, these findings demonstrated that HIF-1ɑ, -2ɑ, and -3ɑ may be important for the development of tibial growth plate to prevent TD and its contribution is to provide new insights on the therapeutic target for poultry TD.
Keywords: Tibetan chickens, growth plate, high altitude, hypoxia inducible factors-1ɑ, tibial dyschondroplasia


© 2017 The Korean Society of Veterinary Science.