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J. Vet. Sci. 2017; 18(S1): 343-349  
Potential biomarkers as an indicator of vertical transmission of Johne’s disease in a Korean native cattle farm
Hong-Tae Park1,†, Hyun-Eui Park1,†, Yong-Il Cho2, Eui-Hyung Kim3, Myunghwan Jung1, Seung Won Shin1, Su-Hyung Lee4, Dae-Yong Kim4, Han Sang Yoo1,*
Departments of 1Infectious Diseases and 4Veterinary Pathology, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea
2Department of Animal Science & Technology, Suncheon National University, Suncheon 57922, Korea
3National Institute of Animal Science, Rural Development Administration, Pyeongchang 25340, Korea
Correspondence to: Tel: +82-2-880-1263; Fax: +82-2-874-2738; E-mail: yoohs@snu.ac.kr
Received: May 25, 2016; Revised: January 19, 2017; Accepted: February 7, 2017; Published online: August 31, 2017.
Abstract
Paratuberculosis (PTB) is caused by Mycobacterium avium subsp. paratuberculosis (MAP) and is one of the most widespread and economically important diseases in cattle. After birth, calves are raised with natural breast feeding without separation from their mothers in most Korean native cattle (Hanwoo breed) farms. Vertical transmission of PTB has been reported, but the exact PTB infection route has not been revealed in Hanwoo farms. Calves of MAP seropositive dams were tested for MAP presence and MAP antibodies in feces and tissues. MAP was detected in calf tissues by using polymerase chain reaction. Expressions of genes reported to be prognostic biomarkers of MAP infection changed in both calves and cows (p < 0.05). Expression of two genes (HGF and SERPINE1) were significantly decreased in MAP-infected cattle and their offspring (p < 0.01). The results suggest that biomarker gene expression profiles can be useful in detecting early stage MAP infection. Based on the results, complete eradication of MAP may be possible if accurate diagnostic methods to detect infected calves are added to the current PTB eradication strategy, which, because infected individuals are likely to develop into fecal MAP shedders at any time, includes isolation of new born calves and feeding sterilized colostrum.
Keywords: Korean native cattle, Mycobacterium avium subsp. paratuberculosis, biomarker, subclinical infection, vertical transmission


© 2017 The Korean Society of Veterinary Science.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.