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J. Vet. Sci. 2017; 18(S1): 361-370  
Generation, characterization, and application in serodiagnosis of recombinant swine vesicular disease virus-like particles
Wanhong Xu, Melissa Goolia, Tim Salo, Zhidong Zhang, Ming Yang*
National Centre for Foreign Animal Disease, Winnipeg, MB R3E 3M4, Canada
Correspondence to: Tel: +1-204-789-2103; Fax: +1-204-789-2038; E-mail: Ming.yang@inspection.gc.ca
Received: July 11, 2016; Revised: December 2, 2016; Accepted: February 22, 2017; Published online: August 31, 2017.
Abstract
Swine vesicular disease (SVD) is a highly contagious viral disease that causes vesicular disease in pigs. The importance of the disease is due to its indistinguishable clinical signs from those of foot-and-mouth disease, which prevents international trade of swine and related products. SVD-specific antibody detection via an enzyme-linked immunosorbent assay (ELISA) is the most versatile and commonly used method for SVD surveillance and export certification. Inactivated SVD virus is the commonly used antigen in SVD-related ELISA. A recombinant SVD virus-like particle (VLP) was generated by using a Bac-to-Bac baculovirus expression system. Results of SVD-VLP analyses from electron microscopy, western blotting, immunofluorescent assay, and mass spectrometry showed that the recombinant SVD-VLP morphologically resemble authentic SVD viruses. The SVD-VLP was evaluated as a replacement for inactivated whole SVD virus in competitive and isotype-specific ELISAs for the detection of antibodies against SVD virus. The recombinant SVD-VLP assay produced results similar to those from inactivated whole virus antigen ELISA. The VLP-based ELISA results were comparable to those from the virus neutralization test for antibody detection in pigs experimentally inoculated with SVD virus. Use of the recombinant SVD-VLP is a safe and valuable alternative to using SVD virus antigen in diagnostic assays.
Keywords: enzyme-linked immunosorbent assay, serological tests, swine vesicular disease virus, virus-like particles


© 2017 The Korean Society of Veterinary Science.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.