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J. Vet. Sci. 2017; 18(S1): 371-379  
Establishment of minimal positive-control conditions to ensure brain safety during rapid development of emergency vaccines
Hyekyung Baek1, Kwang Ho Kim1, Min Young Park1, Kyeongryun Kim1, Bokyeong Ko1, Hyung Seok Seo2, Byoung Soo Kim3,Tae-Wook Hahn4, Sun Shin Yi1,*
1Department of Biomedical Laboratory Science, College of Medical Sciences, Soonchunhyang University, Asan 31538, Korea
2Department of Health Science, Konyang University, Nonsan 32992, Korea
3Department of Molecular Imaging, Korea Institute of Radiology and Medical Sciences, Seoul 01812, Korea
4College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 24341, Korea
Correspondence to: Tel: +82-41-530-4873; Fax: +82-41-530-1085; E-mail: admiral96@sch.ac.kr
Received: April 27, 2017; Revised: June 9, 2017; Accepted: June 21, 2017; Published online: August 31, 2017.
With the increase in international human and material exchanges, contagious and infectious epidemics are occurring. One of the effective methods of epidemic inhibition is the rapid development and supply of vaccines. Considering the safety of the brain during vaccine development is very important. However, manuals for brain safety assays for new vaccines are not uniform or effective globally. Therefore, the aim of this study is to establish a positive-control protocol for an effective brain safety test to enhance rapid vaccine development. The blood-brain barrier’s tight junctions provide selective defense of the brain; however, it is possible to destroy these important microstructures by administering lipopolysaccharides (LPSs), thereby artificially increasing the permeability of brain parenchyma. In this study, test conditions are established so that the degree of brain penetration or brain destruction of newly developed vaccines can be quantitatively identified. The most effective conditions were suggested by measuring time-dependent expressions of tight junction biomarkers (zonula occludens-1 [ZO-1] and occludin) in two types of mice (C57BL/6 and ICR) following exposure to two types of LPS (Salmonella and Escherichia). In the future, we hope that use of the developed positive-control protocol will help speed up the determination of brain safety of novel vaccines.
Keywords: blood-brain barrier, lipopolysaccharides, positive-protocol, tight junctions, vaccine

© 2017 The Korean Society of Veterinary Science.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.