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J Vet Sci 2017; 18(3): 299-306  https://doi.org/10.4142/jvs.2017.18.3.299
Extensive characterization of feline intra-abdominal adipose-derived mesenchymal stem cells
Hee-Ryang Kim1,†, Jienny Lee1,†, Jeong Su Byeon1, Na-Yeon Gu1, Jiyun Lee2, In-Soo Cho1, Sang-Ho Cha1,*
1Viral Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea
2Kangnam Animal Hospital, Pyeongtaek 17982, Korea
Correspondence to: Sang-Ho Cha
Tel: +82-54-912-0805; Fax: +82-54-912-0812; E-mail: virusmania@korea.kr
Received: November 3, 2015; Revised: March 15, 2016; Accepted: June 8, 2016; Published online: September 30, 2017.
Mesenchymal stem cells (MSCs) isolated from various tissues have been well characterized for therapeutic application to clinical diseases. However, in contrast to MSCs from other animal species, the characteristics of feline MSCs have not been fully documented. In this study, we conducted extensive characterization of feline adipose tissue-derived MSCs (fAD-MSCs). Study fAD-MSCs were individually isolated from the intra-abdominal adipose tissues of six felines. The expression levels of cell surface markers and pluripotent markers were evaluated. Next, proliferation capacity was analyzed by performing cumulative population doubling level (CPDL) and doubling time (DT) calculation assays. Differentiation potentials of fAD-MSCs into mesodermal cell lineages were analyzed by examining specific staining and molecular markers. All fAD-MSCs positively expressed cell surface markers such as CD29, CD44, CD90, CD105, CD166, and MHC-I, while CD14, CD34, CD45, and CD73 were negatively expressed. The CPDL of the fAD-MSCs was maintained until passage 5 to 6 (P5 to P6), whereas DT increased after P3 to P4. Also, stem cell-specific pluripotent markers (Oct3/4, Nanog, and SSEA-4) were detected. Importantly, all fAD-MSCs demonstrated mesodermal differentiation capacity. These results suggest that fully characterized fAD-MSCs could be beneficial when considering the use of these cells in feline disease research.
Keywords: adipose tissue-derived mesenchymal stem cells, differentiation, feline, multipotent, proliferation

© 2017 The Korean Society of Veterinary Science.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.