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J Vet Sci 2017; 18(3): 307-316  https://doi.org/10.4142/jvs.2017.18.3.307
Development of an immunochromatographic strip for detection of antibodies against porcine reproductive and respiratory syndrome virus
Huawei Li1,5,†, Jifei Yang2,†, Dengke Bao2, Jie Hou2, Yubao Zhi2, Yanyan Yang2, Pengchao Ji1, Enmin Zhou1, Songlin Qiao2,*, Gaiping Zhang1,3,4,*
1College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China
2Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China
3College of Animal Science and Veterinary Medicine, Henan Agricutural University, Zhenzhou 450002, China
4Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis, Yangzhou 225009, China
5College of Biology Engineering, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China
Correspondence to: Songlin Qiao, Gaiping Zhang
Tel: +86-371-63550369; Fax: +86-371-63558998; E-mails: zhanggaiping2003@163.com (GP Zhang), cdj565@gmail.com (S Qiao)
Received: April 18, 2016; Revised: July 22, 2016; Accepted: November 23, 2016; Published online: September 30, 2017.
Abstract
A simple and rapid immunochromatographic test strip incorporating a colloidal gold-labeled recombinant Nsp7 antigen probe was successfully developed for the detection of anti-porcine reproductive and respiratory syndrome virus (PRRSV) antibodies in swine. Recombinant Nsp7 protein of PRRSV labeled with colloidal gold was dispensed on a conjugate pad for use as the detector. Staphylococcal protein A and purified porcine anti-Nsp7 antibodies were blotted on a nitrocellulose membrane to form test and control lines, respectively. A comparison of the strip with standard diagnostic tests, enzyme-linked immunosorbent assays and immunoperoxidase monolayer assay, was also performed. The immunochromatographic test strip was shown to be of high specificity and sensitivity. Furthermore, the strip assay is rapid and easy to perform with no requirement for professional-level skills or equipment. It is suggested that the immunochromatographic test strip can be used to quickly and accurately detect PRRSV antibody and to be suitable for diagnostic purposes in the field.
Keywords: enzyme-linked immunosorbent assay, immunochromatography, porcine reproductive and respiratory syndrome


© 2017 The Korean Society of Veterinary Science.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.