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J Vet Sci 2017; 18(3): 317-326  https://doi.org/10.4142/jvs.2017.18.3.317
Genetic diversity and phylogenetic analysis of porcine reproductive and respiratory syndrome virus in southern China from 2007 to 2014
Qiwen Wu1, Zhili Li2, Guanqun Zhang3, Jianqiang Niu3, Xiduo Zeng3, Baoli Sun1, Jingyun Ma1,*
1College of Animal Science, South China Agricultural University, Guangzhou 510642, China
2Henan University of Science and Technology, Luoyang 471000, China
3Guangdong Wen’s Foodstuff Group Co., Ltd., Yunfu 527400, China
Correspondence to: Jingyun Ma
Tel/Fax: +86-20-8528-0283; E-mail: majy2400@scau.edu.cn
Received: February 15, 2016; Revised: July 20, 2016; Accepted: November 23, 2016; Published online: September 30, 2017.
Abstract
Porcine reproductive and respiratory syndrome virus (PRRSV) has a high degree of genetic variation. In this study, we characterized the genetic variation and evolutionary relationships among circulating PRRSV strains in southern China. We analyzed 29 NSP2 strains and 150 ORF5 strains from clinical samples collected in southern China during 2007–2014. The alignment results showed that the nucleotide identity similarities of the two genes among these strains were 80.5%–99.7% and 80.9%–100%, respectively. Phylogenetic analysis based on the NSP2 gene showed that highly pathogenic (HP)-PRRSV was still the dominant virus in southern China from 2013 to 2014. Compared with reference strains CH-1a and VR-2332, the field strain 131101-GD-SHC, which shared high homology with JXA1-P170, had a novel 12 amino acid deletion at position 499-510. Phylogenetic analysis based on the ORF5 gene showed that HP-PRRSV, VR2332-like strains, and QYYZ-like strains were simultaneously circulating in southern China from 2007 to 2014, suggesting that, in recent years, the type 2 PRRSV was more diverse in southern China. In conclusion, mutations in the decoy epitope and primary neutralizing epitope could be markers of viral evolution and used to study evolutionary relationships among PRRSV strains in China.
Keywords: GP5, NSP2, phylogenetic analysis, porcine reproductive and respiratory syndrome virus


© 2017 The Korean Society of Veterinary Science.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.