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J Vet Sci 2017; 18(3): 327-332  https://doi.org/10.4142/jvs.2017.18.3.327
Bordetella bronchiseptica antigen enhances the production of Mycoplasma hyopneumoniae antigen-specific immunoglobulin G in mice
Seol-Hwa Yim1, Tae-Wook Hahn2, Hong-Gu Joo1,*
1College of Veterinary Medicine, Jeju National University, Jeju 63243, Korea
2College of Veterinary Medicine, Kangwon National University, Chuncheon 24341, Korea
Correspondence to: Hong-Gu Joo
Tel: +82-64-754-3379; Fax: +82-64-756-3354; E-mail: jooh@jejunu.ac.kr
Received: May 4, 2016; Revised: August 5, 2016; Accepted: September 22, 2016; Published online: September 30, 2017.
Abstract
We previously demonstrated that Bordetella (B.) bronchiseptica antigen (Ag) showed high immunostimulatory effects on mouse bone marrow cells (BMs) while Mycoplasma (M.) hyopneumoniae Ag showed low effects. The focus of this study was to determine if B. bronchiseptica Ag can enhance the M. hyopneumoniae Ag-specific immune response and whether the host’s immune system can recognize both Ags. MTT assay results revealed that each or both Ags did not significantly change BM metabolic activity. Flow cytometry analysis using carboxyfluorescein succinimidyl ester showed that B. bronchiseptica Ag can promote the division of BMs. In cytokine and nitric oxide (NO) assays, B. bronchiseptica Ag boosted production of tumor necrosis factor-alpha in M. hyopneumoniae Ag-treated BMs, and combined treatment with both Ags elevated the level of NO in BMs compared to that from treatment of M. hyopneumoniae Ag alone. Immunoglobulin (Ig)G enzyme-linked immunosorbent assay using the sera of Ag-injected mice clearly indicated that B. bronchiseptica Ag can increase the production of M. hyopneumoniae Ag-specific IgG. This study provided information valuable in the development of M. hyopneumoniae vaccines and showed that B. bronchiseptica Ag can be used both as a vaccine adjuvant and as a vaccine Ag.
Keywords: Bordetella bronchiseptica, Mycoplasma hyopneumoniae, antigen-specific immune response, bone marrow cells, vaccine adjuvant


© 2017 The Korean Society of Veterinary Science.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.