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Integrated analysis of microRNA and mRNA expression in peripheral blood leukocytes of Warmblood horses before and after exercise
Hang-Ah Kim1,3,†, Myung-Chul Kim1,3,†, Na-Yon Kim1,3, Doug-Young Ryu2,4, Hong-Seok Lee1,3, Yongbaek Kim1,4,*
1Laboratory of Veterinary Clinical Pathology, College of Veterinary Medicine, Seoul National University, Seoul, The Republic of Korea 151-742
2Laboratory of Environmental Health and Biomarkers, College of Veterinary Medicine, Seoul National University, Seoul, The Republic of Korea 151-742
3BK21 PLUS Program for Creative Veterinary Science Research, College of Veterinary Medicine, Seoul National University, Seoul, The Republic of Korea 151-742
4Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul, The Republic of Korea 151-742
Correspondence to: Tel: +82-2-880-1273; Fax: +82-2-880-1213; E-mail: yongbaek@snu.ac.kr
These authors contributed equally to this work.
Received: March 9, 2017; Revised: May 29, 2017; Accepted: September 6, 2017; Published online: September 20, 2017.
Abstract
Exercise capacity is a valuable trait in horses, and it has been used as one of their selection criteria. Although exercise affects molecular homeostasis and adaptation in horses, the mechanisms underlying these effects are not fully understood. This study was carried out to identify changes in the blood profiles of microRNAs (miRNAs) and mRNAs induced by exercise in horse leukocytes. Total RNAs isolated from the peripheral blood leukocytes of four Warmblood horses before and after exercise were subjected to next-generation sequencing (NGS) and microarray analyses to determine the miRNA and mRNA expression profiles, respectively. The expressions of 6 miRNAs, including 4 known and 2 novel miRNAs, were altered by exercise. The predicted target genes of the differentially expressed miRNAs identified by NGS were matched to the exercise-induced mRNAs determined by microarray analysis. Five genes, including LOC100050849, LOC100054517, KHDRBS3, LOC100053996, and LOC100062720 from the microarray analysis, were matched to the predicted target genes of the 6 miRNAs. The subset of mRNAs and miRNAs affected by exercise in peripheral blood leukocytes may be useful to expand our understanding about the molecular mechanisms of exercise-associated physiology in horses.
Keywords: Horses; Exercise; Next Generation Sequencing; MicroRNAs; mRNA


© 2017 The Korean Society of Veterinary Science.