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Innate immune response of bovine mammary epithelial cells to Mycoplasma bovis
Satoshi Gondaira1, Hidetoshi Higuchi1,*, Hidetomo Iwano2, Koji Nishi1, Takanori Nebu1, Keiichi Nakajima3, Hajime Nagahata1
1Animal Health Laboratory
2Department of Veterinary Biochemistry, Graduate School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan
3National Agricultural Research Center for Hokkaido Region, National Agriculture and Food Research Organization, Hitsujigaoka, Toyohira-Ku, Sapporo 062-8555, Japan
Correspondence to: Tel: +81-113884844; Fax: +81-113875890; E-mail: higuchi@rakuno.ac.jp
Received: March 23, 2017; Revised: May 29, 2017; Accepted: July 14, 2017; Published online: September 20, 2017.
Abstract
Mycoplasma species are contagious bacteria and Mycoplasma bovis mastitis is a serious productivity problem on dairy farms. Bovine mammary epithelial cells (bMEC) play an important role in the elimination of pathogens, but the effect of M. bovis on bMEC has not been fully clarified. To better understand the immune response against intramammary infection by M. bovis, we used microarray analysis to examine and profile mRNA expression in bMEC after stimulation with M. bovis. We also compared the effects of M. bovis, Staphylococcus aureus and Escherichia coli on immune-related mRNA expression in bMEC. Transcriptome analysis indicated a significant decrease in the level of the mRNA encoding lysine-specific demethylase 4D, suggesting that the immune response is suppressed by a decrease in histone demethylase activity. Interleukin (IL) -1β, IL-6, tumor necrosis factor alpha, Toll-like receptor (TLR) 2 and TLR4 mRNA expression levels were significantly increased in bMEC stimulated with heat-killed (not live) M. bovis, but these expression levels were lower than with stimulation by heat-killed S. aureus or E. coli. Our results suggest that M. bovis only weakly affected mRNA expression in bMEC compared to E. coli or S. aureus. Live M. bovis may induce suppression of immune response in bMEC.
Keywords: Mycoplasma bovis, cytokine mRNA, microarray, bovine mammary epithelial cells, innate immune response


© 2017 The Korean Society of Veterinary Science.