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Recombinant-attenuated Salmonella pullorum strain expressing the hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) protects chickens against NDV and Salmonella Pullorum challenge
Ke Ding, Ke Shang, Zu-Hua Yu, Chuan Yu, Yan-Yan Jia, Lei He, Cheng-Shui Liao, Jing Li, Chun-Jie Zhang, Yin-Ju Li, Ting-Cai Wu, Xiang-Chao Cheng*
Key Laboratory of Animal Disease and Public Health, Henan University of Science and Technology, and Key Laboratory of Live Carrier Biomaterial and Animal Disease Prevention and Control, Luoyang 471003, China
Correspondence to: Tel: +8613303796156; E-mail: chengxch66@163.com
The first authors contributed equally to this work.
Received: June 29, 2017; Revised: September 10, 2017; Accepted: October 7, 2017; Published online: October 13, 2017.
Abstract
Newcastle disease virus (NDV) and Salmonella Pullorum (S. Pullorum) have great damage to the poultry industry, but no perfect vaccine can protect them effectively until now. In this study, a recombinant-attenuated S. Pullorum strain secreting Newcastle disease virus hemagglutinin-neuraminidase (HN) protein, C79-13ΔcrpΔasd (pYA-HN), was constructed as a new bivalent vaccine candidate against Newcastle disease and S. Pullorum disease (PD) by the method of suicide plasmid pREasd-mediated bacteria homologous recombination. The effect of this vaccine candidate was compared with NDV LaSota strain and C79-13ΔcrpΔasd (pYA) strain. The serum HI antibody titers, serum IgG antibodies, the intestinal sIgA and stimulation index in lymphocyte proliferation were significantly higher (P<0.01) induced and increased in chickens inoculated with C79-13ΔcrpΔasd (pYA-HN) strains compared with that of the C79-13ΔcrpΔasd (pYA) strain and were not significantly increased compared with that in the chicken immunized with LaSota live vaccine (P>0.05). And it could provide 60% and 80% protective efficacy against NDV virulent strain F48E9 and S. Pullorum virulent strain C79-13. In summary, the results indicate that recombinant-attenuated S. Pullorum (SP) strain secreting NDV HN protein was constructed, and the generation of C79-13ΔcrpΔasd (pYA-HN) strain provides a good foundation for the development of an effective living-vector double vaccine against NDV and S. Pullorum.
Keywords: Recombinant-attenuated Salmonella Pullorum, Newcastle disease virus, Hemagglutinin-neuraminidase, Vaccine, Immune protective response


© 2017 The Korean Society of Veterinary Science.