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J Vet Sci 2017; 18(4): 547-549  https://doi.org/10.4142/jvs.2017.18.4.547
Surveyor assay to diagnose persistent Müllerian duct syndrome in Miniature Schnauzers
Young June Kim1,†, Hyuk Jin Kwon2, Hyuk Soo Byun2, Donguk Yeom2, Jea-Hong Choi2, Joong-Hyun Kim3, Hosup Shim1,3,4,*
1Department of Nanobiomedical Science and BK21 PLUS NBM Global Research Center for Regenerative Medicine, and 3Institute of Tissue Regeneration Engineering, Dankook University, Cheonan 31116, Korea
2Dream Animal Hospital, Seoul 05570, Korea
4Department of Physiology, Dankook University School of Medicine, Cheonan 31116, Korea
Correspondence to: Hosup Shim
Tel: +82-41-550-3865; Fax: +82-41-559-7839; E-mail: shim@dku.edu
Current address: Institute of Green Bioscience and Technology, Seoul National University, Pyeongchang 25354, Korea
Received: January 4, 2016; Revised: May 3, 2017; Accepted: July 21, 2016; Published online: December 31, 2017.
Abstract
Persistent Müllerian duct syndrome (PMDS) is a pseudohermaphroditism in males characterized by the presence of Müllerian duct derivatives. As PMDS dogs often lack clinical symptoms, a molecular diagnosis is essential to identify the syndrome in these animals. In this study, a new molecular method using DNA mismatch-specific Surveyor nuclease was developed. The Surveyor nuclease assay identified the AMHR2 mutation that produced PMDS in a Miniature Schnauzer as accurately as that obtained by using the conventional method based on restriction digestion. As an alternative to the current molecular diagnostic method, the new method may result in increased accuracy when detecting PMDS.
Keywords: Miniature Schnauzer, persistent Müllerian duct syndrome, surveyor nuclease assay


© 2017 The Korean Society of Veterinary Science.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.