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J Vet Sci 2017; 18(4): 531-540  https://doi.org/10.4142/jvs.2017.18.4.531
Molecular cloning and characterization of porcine ribosomal protein L21
Wu-Sheng Sun1,2, Ju-Lan Chun2, Dong-Hwan Kim1, Jin-Seop Ahn1, Min-Kyu Kim2, In-Sul Hwang3, Dae-Jin Kwon3, Seongsoo Hwang3,*, Jeong-Woong Lee1,*
1Biotherapeutics Translational Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea
2Department of Animal Science and Biotechnology, College of Agriculture and Life Science, Chungnam National University, Daejeon 34134, Korea
3Animal Biotechnology Division, National Institute of Animal Science, Wanju 55365, Korea
Correspondence to: Jeong-Woong Lee, Seongsoo Hwang
Tel: +82-42-860-4428; Fax: +82-42-860-4608; E-mails: jwlee@kribb.re.kr (JW Lee), hwangss@korea.kr (S Hwang)
Received: May 13, 2016; Revised: October 12, 2016; Accepted: November 23, 2016; Published online: December 31, 2017.
Abstract
Ribosomal protein L21 (RPL21) is a structural component of the 60S subunit of the eukaryotic ribosome. This protein has an important role in protein synthesis and the occurrence of hereditary diseases. Pig is a common laboratory model, however, to the best of our knowledge, its RPL21 gene has not been cloned to date. In this study, we cloned and identified the full-length sequence of the pig RPL21 gene for the first time. In addition, we examined its expression pattern and function by using overexpression or knockdown approaches. As a result, we obtained a 604 bp segment that contains a 483 bp open reading frame encoding 160 amino acids. The pig RPL21 gene is located in the “+” strand of chromosome 11, which spans 2167 bp from 4199792 to 4201958. Pig RPL21 protein has nine strands and two helices in its secondary structure. Pig RPL21 is predominantly expressed in ovary and lung, at lower levels in kidney, small intestine, and skin, and at the lowest levels in heart and liver. Furthermore, RPL21 expression is closely connected with cell proliferation and cell cycle arrest. The results are intended to provide useful information for the further study of pig RPL21.
Keywords: Sus scrofa, conservation, gene expression, molecular cloning, ribosomal protein L21


© 2017 The Korean Society of Veterinary Science.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.