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Genomic characterization and pathogenic study of two porcine reproductive and respiratory syndrome viruses with different virulence in Fujian, China
Xiukun Sui1,2,†, Ting Xin1,†, Xiaoyu Guo1, Hong Jia1, Ming Li1, Xintao Gao1, Jing Wu1,2, Yitong Jiang1, Willems L.2, Hongfei Zhu1, Shaohua Hou1,*
1Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences, Beijing 100193, China
2Molecular and Cellular Biology, Gembloux Agro-Bio Tech University of Liège (ULg), 13 Avenue Maréchal Juin, 5030, Gembloux, Belgium
Correspondence to: Tel: +86-15810117467; Fax: +86-10-62815994; E-mail: houshaohua@126.com
The first two authors contributed equally to this article.
Received: August 11, 2017; Revised: October 25, 2017; Accepted: December 26, 2017; Published online: December 28, 2017.
Abstract
Two strains of PRRSVs were isolated in 2006 and 2016, respectively, and designated as FZ06A and FZ16A. Through the inoculation experiments, it was shown that FZ06A caused 100% morbidity and 60% mortality, while FZ16A caused 100% morbidity without death. By using genomic sequence and phylogenetic analyses, close relation of Chinese highly pathogenic PRRSV strain with FZ06A and FZ16A strains was achieved. Based on the achieved results, multiple genomic variations in Nsp2, a unique N-glycosylation site (N33→K33), and a K151 AA substitution for virulence in the GP5 of FZ16A were detected except of 30 amino acids (AA) deletion in the NSP2-coding region. Likewise, inoculation experiments were conducted and weaker virulence of the FZ16A compared to FZ06A strain was obtained. According to the presented data in this article, 30 AA deletion in the NSP2-coding region is not a reliable genomic indicator for the high virulence of PRRSV strains, The difference of Nsp2 and GP5 and the virulence between these two highly pathogenic PRRSV (HP-PRRSV) strains has potential to establish the basis for further studies of virulence determinants for PRRSVs and also to create useful data for development of vaccines against this economically devastating disease.
Keywords: Highly pathogenic porcine reproductive and respiratory syndrome virus; Type 2 PRRSV; Phylogenetic analysis; Pathogenicity


© 2017 The Korean Society of Veterinary Science.