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Clinical assessment after human adipose stem cell transplantation in dogs
Seok Hee Lee1, Erif maha Nugraha Setyawan1, Yoo Bin Choi1, Jeong Chan Ra2, Sung Keun Kang2, Geon A Kim1*, Byeong Chun Lee1,*
1Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 08826, Republic of Korea
2Biostar Stem Cell Research Institute, R Bio Co., Ltd., Seoul 08506, Republic of Korea
Correspondence to: Tel: +82-2-880-1269; Fax: +82-2-873-1269; E-mail: bclee@snu.ac.kr, pshsje03@snu.ac.kr
Received: October 10, 2017; Revised: December 15, 2017; Accepted: December 26, 2017; Published online: December 28, 2017.
Abstract
Adipose stem cells (ASCs) are an attractive source of stem cells with therapeutic applicability in various fields for regenerating damaged tissues because they show stemness characteristics. However, little investigation has been done to evaluate adverse responses caused by human ASCs therapy. Therefore, in the present study, a clinical assessment after human ASCs transplantation in dogs was investigated.
A total of 12 healthy male dogs were selected and divided into 4 groups: 1) saline infusion and bolus groups, and 2) ASCs infusion and bolus groups. Physical assessment and blood analysis were performed following ASCs transplantation, and the concentration of angiogenic factors, and pro- and anti-inflammatory cytokines was measured by enzyme-linked immunosorbent assay.
There were no adverse responses in vital signs among the dogs. In blood analysis, there were no remarkable findings in CBC and serum chemistry parameters. In ELISA assay, level of angiogenic and anti-inflammatory factors including MMP9, VEGF, bFGF, HGF and IL-10 were significantly increased in ASCs groups compared with control.
In conclusion, this study demonstrated that transplantation of human ASCs showed no adverse effects and could be used in dogs. Also, human ASCs could be involved in modulating secretion of angiogenic factors including MMP9, VEGF, bFGF, HGF and anti-inflammatory factor IL-10.
Keywords: Dogs; Adipose stem cells; Transplantation; Angiogenic factors; Anti-inflammatory factor


© 2017 The Korean Society of Veterinary Science.