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Dembo-PCR technique for the detection of bovine abortion, diarrhea, and respiratory disease complex infectious agents in potential vectors and reservoirs
Sayed Samim Rahpaya1,2,9,†, Shinobu Tsuchiaka1,2,†, Mai Kishimoto1, Mami Oba1, Yukie Katayama1, Yuki Nunomura1, Saki Kokawa1, Takashi Kimura3, Atsushi Kobayashi3, Yumi Kirino4, Tamaki Okabayashi4, Nariaki Nonaka4, Hirohisa Mekata4, Hiroshi Aoki5, Mai Shiokawa5, Moeko Umetsu5, Tatsushi Morita5, Ayako Hasebe6, Keiko Otsu6, Tetsuo Asai2, 6 Tomohiro Yamaguchi7, Shinji Makino8, Yoshiteru Murata1, Ahmad Jan Abi9, Tsutomu Omatsu1,2, Tetsuya Mizutani1,2,*
1Research and Education Center for Prevention of Global Infectious Diseases of Animals, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 183-0045 Tokyo, Japan
2United Graduate School of Veterinary Science, Gifu University, 501-1193 Gifu, Japan
3Laboratory of Comparative Pathology, Department of Clinical Science, Faculty of Veterinary Medicine, Hokkaido University, 060-0808 Sapporo, Japan
4Center for Animal Disease Control, University of Miyazaki, 889-2192 Miyazaki, Japan
5Faculty of Veterinary Science, Nippon Veterinary and Life Science University, 180-8602 Tokyo, Japan.
6Education and Research Center for Food Animal Health (GeFAH), Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan
7Canine-Lab. Inc., 184–0012 Tokyo, Japan
8Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, TX 77555-1019 Texas, U.S.A
9Faculty of Veterinary Science, Department of Paraclinic, Kabul University, 3rd district Kabul, Afghanistan
Correspondence to: Tel: +81-42-367-5749; Fax: 81-42-367-5742; E-mail: tmizutan@cc.tuat.ac.jp
The first two authors contributed equally to this work.
Received: October 30, 2017; Revised: December 12, 2017; Accepted: December 26, 2017; Published online: December 28, 2017.
The bovine abortion, diarrhea, and respiratory disease complexes, caused by infectious agents, result in high and significant economic losses for the cattle industry. These pathogens are likely transmitted by various vectors and reservoirs including insects, birds and rodents. However, experimental data that supports this possibility are scarce. We collected total 117 samples and screened for 44 bovine abortive, diarrheal and respiratory disease complex pathogens by using Dembo-PCR, based on TaqMan real-time PCR. Fifty-seven samples showed positive for at least one pathogen, including bovine viral diarrhea virus, bovine enterovirus, Salmonella enterica ser. Dublin, salmonella enterica ser. Typhimurium, and Neospora caninum; some samples contained multiple pathogens. Bovine viral diarrhea virus and bovine enterovirus represented the most frequently detected pathogens, especially in flies, suggesting the important role of flies in the transmission of these viruses. Additionally, we detected the N. caninum genome from a cockroach sample for the first time. Our data suggests that insects (particularly flies), birds, and rodents are potential vectors and reservoirs of the abortion, diarrhea, and respiratory infectious agents and that they may transmit more than one pathogen at the same time.
Keywords: Cattle, Dembo-PCR, Pathogen, Reservoir, Vector

© 2017 The Korean Society of Veterinary Science.