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J Vet Sci 2018; 19(1): 79-87  https://doi.org/10.4142/jvs.2018.19.1.79
Innate immune response of bovine mammary epithelial cells to Mycoplasma bovis
Satoshi Gondaira1, Hidetoshi Higuchi1,*, Hidetomo Iwano2, Koji Nishi1, Takanori Nebu1, Keiichi Nakajima3, Hajime Nagahata1
1Animal Health Laboratory, and 2Department of Veterinary Biochemistry, Graduate School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu 069-8501, Japan
3National Agricultural Research Center for Hokkaido Region, National Agriculture and Food Research Organization, Sapporo 062-8555, Japan
Correspondence to: Hidetoshi Higuchi
Tel: +81-113884844; Fax: +81-113875890; E-mail: higuchi@rakuno.ac.jp
Received: March 23, 2017; Revised: May 29, 2017; Accepted: July 14, 2017; Published online: January 31, 2018.
Mycoplasma spp. are contagious bacteria, and mycoplasmal mastitis is a serious productivity problem on dairy farms. Bovine mammary epithelial cells (bMECs) have an important role in the elimination of pathogens, but the effect of Mycoplasma bovis on bMECs has not been fully described. To elucidate the immune response against intramammary infection by M. bovis, we undertook microarray analysis to examine and profile mRNA expression in bMECs after stimulation with M. bovis. We also compared the effects of M. bovis, Staphylococcus aureus, and Escherichia coli on immune-related mRNA expression in bMECs. Transcriptome analysis indicated a significant decrease in the level of mRNA-encoding lysine-specific demethylase 4D, suggesting that the immune response is suppressed by a decrease in histone demethylase activity. Interleukin (IL)-1β, IL-6, tumor necrosis factor alpha, toll-like receptor (TLR) 2, and TLR4 mRNA expression levels were significantly increased in bMECs stimulated with heat-killed M. bovis, but the expression levels were lower than those following stimulation by heat-killed S. aureus or E. coli. Our results suggest that M. bovis weakly affects mRNA expression in bMECs compared to the effects of E. coli or S. aureus. Moreover, live M. bovis may induce suppression of the immune response in bMECs.
Keywords: Mycoplasma bovis, bovine mammary epithelial cells, cytokines, innate immunity, microarray analysis

© 2018 The Korean Society of Veterinary Science.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.