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J Vet Sci 2018; 19(1): 89-98  https://doi.org/10.4142/jvs.2018.19.1.89
A new recombined porcine reproductive and respiratory syndrome virus virulent strain in China
Jian-guo Dong1,2,†, Lin-yang Yu1,†, Pei-pei Wang1, Le-yi Zhang1, Yan-ling Liu1, Peng-shuai Liang1, Chang-xu Song1,*
1Animal Science College & National Engineering Center for Swine Breeding Industry, South China Agriculture University, Guangzhou 510642, China
2School of Animal Husbandry and Medical Engineering, Xinyang Agriculture and Forestry University, Xinyang 464000, China
Correspondence to: Chang-xu Song
Tel: +86-13829723528; E-mail: cxsong2004@163.com
Received: December 19, 2016; Revised: June 2, 2017; Accepted: June 29, 2017; Published online: January 31, 2018.
Abstract
Porcine reproductive and respiratory syndrome (PRRS) is one of the most important swine diseases worldwide. In the present study, a new virulent strain of PRRS virus (PRRSV), GDsg, was isolated in Guangdong province, China, and caused high fever, high morbidity, and high mortality in sows and piglets. The genome of this new strain was 15,413 nucleotides (nt) long, and comparative analysis revealed that GDsg shared 82.4% to 94% identity with type 2 PRRSV strains, but only 61.5% identity with type 1 PRRSV Lelystad virus strain. Phylogenetic analysis indicated that type 2 PRRSV isolates include five subgenotypes (I, II, III, IV, and V), which are represented by NADC30, VR-2332, GM2, CH-1a, and HuN4, respectively. Moreover, GDsg belongs to a newly emerging type 2 PRRSV subgenotype III. More interestingly, the newly isolated GDsg strain has multiple discontinuous nt deletions, 131 (19 + 18 + 94) at position 1404–1540 and a 107 nt insertion in the NSP2 region. Most importantly, the GDsg strain was identified as a virus recombined between low pathogenic field strain QYYZ and vaccine strain JXA1-P80. In conclusion, a new independent subgenotype and recombinant PRRSV strain has emerged in China and could be a new threat to the swine industry of China.
Keywords: new subgenotype, phylogenetic analysis, porcine reproductive and respiratory syndrome virus, recombinant strain


© 2018 The Korean Society of Veterinary Science.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.