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J Vet Sci 2018; 19(1): 99-106  https://doi.org/10.4142/jvs.2018.19.1.99
Integrated analysis of microRNA and mRNA expressions in peripheral blood leukocytes of Warmblood horses before and after exercise
Hang-Ah Kim1,3,†, Myung-Chul Kim1,3,†, Na-Yon Kim1,3, Doug-Young Ryu2,4, Hong-Seok Lee1,3, Yongbaek Kim1,4,*
1Laboratory of Veterinary Clinical Pathology, 2Laboratory of Environmental Health and Biomarkers, 3BK21 PLUS Program for Creative Veterinary Science Research, and 4Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea
Correspondence to: Yongbaek Kim
Tel: +82-2-880-1273; Fax: +82-2-880-1213; E-mail: yongbaek@snu.ac.kr
The first two authors contributed equally to this work.
Received: March 9, 2017; Revised: May 29, 2017; Accepted: September 6, 2017; Published online: January 31, 2018.
Abstract
Exercise capacity is a valuable trait in horses, and it has been used as a horse selection criterion. Although exercise affects molecular homeostasis and adaptation in horses, the mechanisms underlying these effects are not fully described. This study was carried out to identify changes in the blood profiles of microRNAs (miRNAs) and mRNAs induced by exercise in horse leukocytes. Total RNAs isolated from the peripheral blood leukocytes of four Warmblood horses before and after exercise were subjected to next-generation sequencing (NGS) and microarray analyses to determine the miRNA and mRNA expression profiles, respectively. The expressions of 6 miRNAs, including 4 known and 2 novel miRNAs, were altered by exercise. The predicted target genes of the differentially expressed miRNAs identified by NGS were matched to the exercise-induced mRNAs determined by microarray analysis. Five genes (LOC100050849, LOC100054517, KHDRBS3, LOC100053996, and LOC100062720) from the microarray analysis were matched to the predicted target genes of the 6 miRNAs. The subset of mRNAs and miRNAs affected by exercise in peripheral blood leukocytes may be useful in elucidating the molecular mechanisms of exercise-associated physiology in horses.
Keywords: exercise, horses, messenger RNA, microRNAs, next-generation sequencing


© 2018 The Korean Society of Veterinary Science.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.