• home
  • articles
  • authors
  • Reviewers
  • About the Journal
  • About the Journal
  • About the Journal
  • About the Journal
  • e-Submission

Indexed/Covered by

J Vet Sci 2018; 19(1): 107-115  https://doi.org/10.4142/jvs.2018.19.1.107
Tibial dyschondroplasia is closely related to suppression of expression of hypoxia-inducible factors 1α, 2α, and 3α in chickens
Shucheng Huang1, Mujeeb U. Rehman1, Gang Qiu1,2, Houqiang Luo1,3, Muhammad K. Iqbal1, Hui Zhang1, Khalid Mehmood1,†, Jiakui Li1,2,*
1College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China
2Laboratory of Detection and Monitoring of Highland Animal Disease, Tibet Agriculture and Animal Husbandry College, Linzhi 860000, Tibet, China
3Animal Science Department, Wenzhou Vocational College of Science and Technology, Wenzhou 325006, China
Correspondence to: Jiakui Li
Tel: +86-27-87286251; E-mail: lijk210@sina.com
Present address: University College of Veterinary and Animal Sciences, The Islamia University of Bahawalpur 63100, Pakistan
Received: February 7, 2017; Revised: April 4, 2017; Accepted: May 5, 2017; Published online: January 31, 2018.
Abstract
Tibial dyschondroplasia (TD) cases has not been reported in Tibetan chickens (TBCs), but it is commonly seen in commercial broilers characterized by lameness. The underlying mechanism remains unclear. Hypoxia-inducible factors (HIFs) are important regulators of cellular adaptation to hypoxic conditions. In this study, we investigated the role of HIF-1α, -2α, and -3α in hypoxia and thiram-induced TD and their effect on tibial growth plate development in Arbor Acres chickens (AACs) and TBCs. RNA and protein expression levels of HIF-1α, -2α, and -3α were determined by using quantitative reverse transcriptase polymerase chain reaction and western blotting analyses, respectively. Interestingly, the results showed that HIF-1α, -2α, and -3α expressions in the tibial growth plate of TBCs were upregulated by hypoxia and the change was more significant in TBCs than in AACs. However, these factors were downregulated in thiram-induced TD. To further clarify the effect of thiram on tibial growth plate in commercial broilers, AACs were observed to exhibit more pronounced changes in their growth plate that that in TBCs. Taken together, these results demonstrate that HIF-1α, -2α, and -3α may be important in tibial growth plate development and in the prevention of TD. The present study contributes novel insights on a therapeutic target for poultry TD.
Keywords: Tibetan chickens, growth plate, high altitude, hypoxia-inducible factors-1α, tibial dyschondroplasia


© 2018 The Korean Society of Veterinary Science.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.