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Identification, sequence analysis, and infectivity of H9N2 avian influenza viruses isolated from geese
Rui Zhu1,2,†, Xueqin Yang1,2,†, Jianjun Zhang3, Danwen Xu1,2, Jiawen Fan1,2, Huoying Shi1,2,*, Shifeng Wang4, Xiufan Liu1,2
1College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, China
2Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China
3Sinopharm Yangzhou VAC Biological Engineering Co., Ltd., Yangzhou, Jiangsu, PR China.
4Department of Infectious Diseases and Pathology, College of Veterinary Medicine, University of Florida, Gainesville, FL 32611-0880, USA
Correspondence to: Tel: +86-514-87977968; Fax: +86-514-87972218; E-mail: hyshi@yzu.edu.cn
These authors contributed equally to this work.
Received: May 24, 2017; Revised: December 9, 2017; Accepted: December 29, 2017; Published online: January 25, 2018.
Abstract
H9N2 subtype avian influenza virus is a great threat to the Chinese poultry industry, even with annual vaccination. Waterfowl can be asymptomatically infected with the H9N2 virus. In this study, three strains of the H9N2 avian influenza virus, designated A/Goose/Jiangsu/YZ527/2011 (H9N2, Gs/JS/YZ527/11), A/Goose/Jiangsu/SQ119/2012 (H9N2, Gs/JS/SQ119/12), and A/Goose/Jiangsu/JD564/2012 (H9N2, Gs/JS/JD564/12), were isolated from domestic geese. Molecular characterization of the three isolates showed that the Gs/JS/YZ527/11 virus is a double-reassortant virus, combining the genes of A/Quail/Hong Kong/G1/97 (H9N2, G1/97) - like and A/Chicken/Shanghai/F/98 (H9N2, F/98) - like; the Gs/JS/SQ119/12 virus is a triple-reassortant virus derived from G1/97 - like, F/98 - like, and A/Duck/Shantou/163/2004 (H9N2, ST/163/04) - like. The sequences of the Gs/JS/JD564/12 virus share high homology with those of the F/98 virus, except for the neuraminidase (NA) gene, and the internal genes of the Gs/JS/YZ527/11 and Gs/JS/SQ119/12 viruses are closely related to those of the H7N9 viruses. An infectivity analysis of the three isolated strains showed that Gs/JS/SQ119/12 and Gs/JS/YZ527/11 replicated well, with seroconversion, in geese and chickens, whereas Gs/JS/JD564/12 was not well infected to either geese or chickens and the F/98 virus only infected chickens, with seroconversion. The emergence of these new reassortant H9N2 influenza viruses indicates that these viruses have infected both chicken and goose with a few types of lesions.
Keywords: H9N2, goose, chicken, biological characteristics, infectivity


© 2018 The Korean Society of Veterinary Science.