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Analysis of Swine Leukocyte Antigen (SLA) class I genes profiles and PERV viremia level in the transgenic porcine herd inbred for xenotransplantation research
Daria Matczyńska1,*, Daniel Sypniewski1, Sabina Gałka1, Dagna Sołtysik1, Tomasz Loch1, Ewa Nowak1, Zdzisław Smorąg2, Ilona Bednarek1
1Department of Biotechnology and Genetic Engineering, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia in Katowice, Jednosci 8, 41-200 Sosnowiec, Poland
2Department of Animal Reproduction Biotechnology, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland
Correspondence to: Tel: +48-32-364-1274; Fax: +48-32-364-1276; E-mail: dmatczynska@sum.edu.pl
Received: August 3, 2017; Revised: December 29, 2017; Accepted: January 20, 2018; Published online: January 25, 2018.
Molecular characterization of the SLA (Swine Leukocyte Antigen) genes is important for understanding the immune responses between swine-donor and human-recipient in course of xenotransplantation. Explanation of association between alleles of SLA class I genes, type of pigs genetic modification, the PERV viral titer, and its subtypes may shed light on the nature of xenograft acceptance or rejection and the safety of xenotransplantation. No significant differences in PERV gag RNA level between transgenic and non-transgenic pigs were noted, likewise the type of applied transgene had no impact on PERV viremia. Type of SLA-1 gene profile may correspond with PERVs level in blood and thereby influence on their infectiveness. Screening tests of pigs should also enable selection of animals with low expression of PERV and exclusion specimens with PERV-C in the genome due to possible recombination between A and C subtypes, which may lead to autoinfection. 31.25% of study specimens shows the presence of PERV-C integrated in genome, but statistically significant increased viremia, in regard to specimens with PERV-C, was not observed. There is a need for multidirectional molecular characterization (SLA typing, viremia estimation and PERV subtypes screening) of animals intended for xenotransplant research in the interest of xeno-recipients safety.
Keywords: swine leukocyte antigen, xenotransplantation, retroviruses, viremia, infection

© 2018 The Korean Society of Veterinary Science.