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Marek’s disease vaccine activates chicken macrophages
Dan Wang1, Shuhong Sun1, Mohammad Heidari2,*
1College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an, Shandong Province, China
2United States Department of Agriculture, Agriculture Research Service, Avian Disease and Oncology Laboratory, East Lansing, MI 48823, USA
Correspondence to: Tel: +1-517-337-6830; Fax: +1-517-337-6776; E-mail: mohammad.heidari@ars.usda.gov
Received: October 10, 2017; Revised: January 5, 2018; Accepted: January 10, 2018; Published online: January 25, 2018.
Abstract
To provide insights into the role of innate immune responses in vaccine-mediated protection, we investigated the effect of Marek’s disease (MD) vaccine, Rispens/CVI988, on the expression pattern of selected genes associated with activation of macrophages in MD-resistant and susceptible chicken lines. Upregulation of IFN-γ, IL-1β, IL-8, and IL-12 at different time points post inoculation (dpi) revealed activation of macrophages in both chicken lines. Strong immune response was induced in cecal tonsils of the susceptible line at 5 dpi. The highest transcriptional activities were observed in the spleen tissues of the resistant line at 3 dpi. No increase in the population of CD3+ T cells was observed in duodenum of vaccinated birds at 5 dpi indicating the lack of involvement of adaptive immune system in the transcriptional profiling of the tested genes. There was, however, an increase in the number of macrophages in the duodenum of vaccinated birds. The CVI988/Rispens antigen was detected in the duodenum and cecal tonsils of the susceptible line but not the resistant line at 5 dpi. This study sheds light on the role of macrophages in vaccine-mediated protection against MD and possible development of new recombinant vaccines with enhanced innate immune system activation properties.
Keywords: Marek’s disease, CVI988/Rispens, macrophage, duodenum, cecal tonsils


© 2018 The Korean Society of Veterinary Science.