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Effect of immunization routes and protective efficacy of Brucella antigens delivered via Salmonella vector vaccine
Jonathan Lalsiamthara, Gayeon Won, John Hwa Lee*
College of Veterinary Medicine, Chonbuk National University, Iksan Campus, 570-752, Iksan, Republic of Korea
Correspondence to: Tel.: +82-63-850-0940; Fax: +82-63-850-0910; E-mail: johnhlee@jbnu.ac.kr
Received: October 24, 2017; Revised: December 4, 2017; Accepted: January 10, 2018; Published online: January 25, 2018.
An anti-Brucella vaccine candidate which comprises of purified Brucella lipopolysaccharide and cocktail of four Salmonella Typhimurium (ST)-Brucella vectors was reported earlier. Each vector constitutively express highly conserved Brucella antigens (rB), viz. lumazine synthase (BLS), proline racemase subunit A (PrpA), outer membrane protein-19 (Omp19), and Cu-Zn superoxide dismutase (SOD). The present study determined relative level of protection conferred by each single strain. Upon virulent challenge, the challenge strain was recovered most abundantly in non-immunized control mice and the ST-Omp19, ST-BLS, LPS, and ST-SOD immunized mice groups showed much less burden. Indirect ELISA based assay also confirmed the induction of antigen specific IgG for each antigen delivered. In route-wise comparison of the combined vaccine candidate, intraperitoneal (IP), intramuscular (IM), and subcutaneous (SC) immunizations revealed an indication of highly efficient routes for the protection. Splenocytes of mice immunized via IM and IP route induce significant relative expression of IL-17 upon antigenic pulsing. Taken together, each of Brucella antigens delivered by ST successfully induced antigen specific immune response and it was also evident that individual antigen strain can also confer considerable degree of protection. More effective protection was observed when the candidate was inoculated via intraperitoneal (IP) and intramuscular (IM) routes.
Keywords: vaccine; protective efficacy; Salmonella delivery; brucellosis

© 2018 The Korean Society of Veterinary Science.