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Establishment and identification of cell lines from type O blood Korean native pigs and their efficiency in supporting embryonic development via somatic cell nuclear transfer
Anukul Taweechaipaisankul1,†, Geon A Kim1,†, Jun-Xue Jin1, Su Cheong Yeom2, Byeong Chun Lee1,2,*
1Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea
2Institutes of Green Bio Science and Technology, Seoul National University, Pyeongchang 25354, Korea
Correspondence to: Tel: +82-2-880-1229; Fax: +82-2-873-1269; E-mail: bclee@snu.ac.kr
The first two authors contributed equally to this work.
Received: March 10, 2017; Revised: July 28, 2017; Accepted: February 2, 2018; Published online: February 27, 2018.
Due to their close similarities with humans in anatomy, physiology and genetics and handling advantages, miniature pigs are becoming a very attractive model for biomedical research. The purpose of this study was to establish and evaluate blood type O cells derived from Korean native pig (KNP), a typical miniature pig breed in Korea. Total 10 cell lines derived from 8 KNP piglets, adult KNP female pig (kidney and ear tissues) were established. To confirm the blood type O, genomic DNA, fucosyltransferase (FUT) expression and immunofluorescence staining were examined. Additionally, Fluorescence-activated cell sorting and somatic cell nuclear transfer were performed to investigate the normality of cell lines and to evaluate their efficiency on embryo development. We found no significant bands corresponding to specific blood group A, expression of FUT in cell lines derived from piglets #1, #4, #5, #8 and adult female KNP. Moreover, they showed normal expression of α 1,3-galactosyltransferase (alpha-Gal) and cytidine monophospho-N-acetylneuraminic acid hydroxylase (CMAH). However, there was no significant difference in embryo development between skin and kidney fibroblasts derived from blood type O KNP. In conclusion, we successfully established the blood type O KNP cell lines, which may serve as a useful model for xenotransplantation research.
Keywords: Korean native pig, Miniature pig, Somatic cell nuclear transfer, Xenotransplantation

© 2018 The Korean Society of Veterinary Science.