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Prevalence, toxin gene profile, antibiotic resistance, and molecular characterization of Clostridium perfringens from diarrheic and non-diarrheic dogs in South Korea
Jung-Whan Chon1, Kun-Ho Seo2, Dongryeoul Bae1, Ji-Hee Park2, Saeed Khan1, Kidon Sung1,*
1Division of Microbiology, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079, USA
2KU Center for One Health, College of Veterinary Medicine, Konkuk University, Seoul 05029, Republic of Korea
Correspondence to: Tel: +1-870-543-7527; Fax: +1-870-543-7307; Email: Kidon.Sung@fda.hhs.gov
Received: June 12, 2017; Revised: January 18, 2018; Accepted: February 10, 2018; Published online: February 27, 2018.
Clostridium perfringens causes diarrhea and other diseases in animals and humans. We investigated the prevalence, toxin gene profiles, and antibiotic resistance of C. perfringens isolated from diarrheic and non-diarrheic dogs in two animal hospitals in Seoul, South Korea. Fecal samples were collected from clinically diarrheic (DD; n=49) and non-diarrheic dogs (ND; n=34). C. perfringens was isolated from 31 out of 49 DD (63%) and 21 out of 34 ND dogs (62%). All strains were positive for the α toxin gene, but not for the β, ε, and ι toxin genes; therefore, all the strains were identified as type A C. perfringens. All the isolates were cpe-negative, whereas the β2 toxin gene was identified in 84% and 62% of isolates from DD and ND, respectively. Most isolates were susceptible to ampicillin (94%), chloramphenicol (92%), metronidazole (100%), moxifloxacin (96%), and imipenem (100%). However, 25% and 21% of isolates were resistant to tetracycline and clindamycin, respectively. Molecular subtyping of the isolated strains was performed by pulsed-field gel electrophoresis (PFGE). Fifty-two isolates were classified into 48 pulsotypes based on more than 90% similarity of the banding patterns. No noticeable difference was observed between the isolates from DD and ND.
Keywords: C. perfringens, dog, toxin, resistance, PFGE

© 2018 The Korean Society of Veterinary Science.