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Effect of superoxide dismutase, catalase and glutathione peroxidase supplementation in the extender on chilled semen of fertile and hypofertile dogs
Chiara Del Prete1,*, Francesca Ciani1,*, Simona Tafuri1, Maria Pia Pasolini1, Giovanni Della Valle1, Veronica Palumbo1, Lucia Abbondante2, Antonio Calamo1, Vincenza Barbato3, Roberto Gualtieri3, Riccardo Talevi3, Natascia Cocchia1
1Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Via a Federico Delpino, 1-80137-Napoli, Italy
2Freelancer, Naples, Italy
3Department of Biology, University of Naples Federico II, Via Cinthia-80126-Napoli, Italy
Correspondence to: Tel: +39-81-2536017; E-mail: chiara.delprete@unina.it; ciani@unina.it.
Received: August 17, 2017; Revised: March 23, 2018; Accepted: April 4, 2018; Published online: April 12, 2018.
This study investigated the correlation between oxidative stress status and main canine sperm parameters and the effect of addition of Superoxide Dismutase (SOD), Catalase (CAT) and Glutathione Peroxidase (GPx) combination in egg yolk tris-citrate glucose (EYT-G) extender on semen during 10 days of storage at 4° C. Ten Boxer dogs were included in this study and divided in two groups, fertile (F) and hypofertile (H), depending on pregnancy and live birth rates in the previous year. The semen evaluation was performed at the day of collection (D0) and after 5 (D5) and 10 (D10) days of cooled storage at 5° C. Sperm motility, kinetic parameters and DNA integrity were assessed. A correlation between oxidative status and the main semen parameters in both F and H groups was found. Total and progressive motility were significantly higher in treated (SOD, CAT and GPx addition) versus control groups at D10 in both F and H groups, and at D5 in H group. DNA integrity was significantly higher in both treated groups (H and F) at D5 and D10. In conclusion, the addition of SOD, CAT and GPx in the extender has allowed the preservation of semen quality up to 10 days of storage at 5° C both in fertile and hypofertile dogs.
Keywords: chilled semen, antioxidants, semen preservation, extender, canine

© 2018 The Korean Society of Veterinary Science.