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A Salmonella Typhi ghost induced by the E gene of phage φX174 stimulates dendritic cells and efficiently activates the adaptive immune response
Gayeon Won, Seong Kug Eo, Sang-Youel Park, Jin Hur, John Hwa Lee*
College of Veterinary Medicine, Chonbuk National University, Iksan campus, Republic of Korea
Correspondence to: Tel: +82-63-850-0940; Fax: +82-63-850-0910; E-mail: johnhlee@jbnu.ac.kr
Received: November 14, 2017; Revised: February 2, 2018; Accepted: March 7, 2018; Published online: April 12, 2018.
Abstract
We previously genetically engineered a Salmonella Typhi bacterial ghost (STG) as a novel inactivated vaccine candidate against typhoid fever. The underlying mechanism employed by the ghost to stimulate the adaptive immune response remains to be investigated. In this study, we aimed to evaluate the immunostimulatory effect of STG on mouse bone marrow-derived dendritic cells (BMDCs) and its activation of the adaptive immune response in vitro. Immature BMDCs were stimulated with STG, which efficiently stimulated maturation events in BMDCs, as indicated by up-regulated expression of CD40, CD80, and MHC-II molecules on CD11+ BMDCs. Immature BMDCs responded to stimulation with STG by significantly increasing the expression of IL-6, which might indicate the induction of DC maturation in vivo (P < 0.05). In addition, ghost-stimulated murine BMDCs showed significant expression of IFN-γ and IL-4. This expression can drive the development of Th1 and Th2 cells, respectively, in co-cultured CD4+ T cells in vitro. These findings suggest that STG can effectively stimulate maturation of BMDCs and facilitate subsequent immune responses via potent immunomodulatory cytokine responses.
Keywords: dendritic cells, innate immunity, salmonella typhi


© 2018 The Korean Society of Veterinary Science.