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Sciatic nerve leachate of cattle causes neuronal differentiation of PC12 cells via ERK1/2 signaling pathway
Ziqiang Zhang, Yumei Liu*, Xuemin Zhu, Lan Wei, Jiamin Zhu,Ke Shi, Guotao Wang, Li Pan
College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan 471023 China
Correspondence to: Tel: +86-15324617957; Fax: +86-37964563979; E-mail: lymzq@126.com
Received: March 29, 2018; Accepted: April 23, 2018; Published online: April 26, 2018.
Abstract
Previous studies have shown that sciatic nerve has neurotrophic activity, and nerve regeneration, differentiation, axon outgrowth can be modulated by different sciatic nerve preparations. However, a number of animals have to be sacrificed to get sciatic nerves for making sciatic nerve preparations. Some studies have demonstrated that the role of sciatic nerve preparations in neural differentiation depends on the neurotrophins that schwann cells secrete, and these factors are highly conserved between different species. To save experimental animals, in this study, we made sciatic nerve leachate of cattle and explored its effect on neuronal differentiation of rat PC12 cells (a useful model for studying neuronal differentiation). Results showed the neurite outgrowth of PC12 cells treated with sciatic nerve leachate of cattle for 3, 6, 9 days was significantly improved, and the expression of β3-tubulin, microtubule-associated protein 2 (MAP2) (two neuron-specific protein) was increased. The ERK1/2 signaling pathway was activated after PC12 cells were incubated with sciatic nerve leachate of cattle for 9 days. Thus sciatic nerve leachate of cattle can effectively induce neuronal differentiation of rat PC12 cells via ERK1/2 signaling pathway.
Keywords: Sciatic nerve, Schwann cells, PC12 cells, Neuronal differentiation


© 2018 The Korean Society of Veterinary Science.