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J Vet Sci 2018; 19(3): 339-349  https://doi.org/10.4142/jvs.2018.19.3.339
Genomic characterization and pathogenic study of two porcine reproductive and respiratory syndrome viruses with different virulence in Fujian, China
Xiukun Sui1,2,†, Ting Xin1,†, Xiaoyu Guo1, Hong Jia1, Ming Li1, Xintao Gao1, Jing Wu1,2, Yitong Jiang1, L.Willems2, Hongfei Zhu1, Shaohua Hou1,*
1Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
2Molecular and Cellular Biology, Gembloux Agro-Bio Tech University of Liège, 5030 Gembloux, Belgium
Correspondence to: Tel: +86-15810117467; Fax: +86-10-62815994; E-mail: houshaohua@126.com
The first two authors contributed equally to this work.
Received: August 11, 2017; Revised: October 25, 2017; Accepted: December 26, 2017; Published online: May 31, 2018.
Two strains of porcine reproductive and respiratory syndrome virus (PRRSV) were isolated in 2006 and 2016 and designated as FZ06A and FZ16A, respectively. Inoculation experiments showed that FZ06A caused 100% morbidity and 60% mortality, while FZ16A caused 100% morbidity without death. By using genomic sequence and phylogenetic analyses, close relationships between a Chinese highly pathogenic PRRSV strain and the FZ06A and FZ16A strains were observed. Based on the achieved results, multiple genomic variations in Nsp2, a unique N-glycosylation site (N33→K33), and a K151 amino acid (AA) substitution for virulence in the GP5 of FZ16A were detected; except the 30 AA deletion in the Nsp2-coding region. Inoculation experiments were conducted and weaker virulence of FZ16A than FZ06A was observed. Based on our results, a 30 AA deletion in the Nsp2-coding region is an unreliable genomic indicator of a high virulence PRRSV strain. The Nsp2 and GP5 differences, in addition to the virulence difference between these two highly pathogenic PRRSV strains, have the potential to be used to establish a basis for further study of PRRSV virulence determinants and to provide data useful in the development of vaccines against this economically devastating disease.
Keywords: genomics, phylogeny, porcine respiratory and reproductive syndrome virus, virulence

© 2018 The Korean Society of Veterinary Science.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.