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J Vet Sci 2018; 19(3): 368-374  https://doi.org/10.4142/jvs.2018.19.3.368
Prevalence, toxin gene profile, antibiotic resistance, and molecular characterization of Clostridium perfringens from diarrheic and non-diarrheic dogs in Korea
Jung-Whan Chon1, Kun-Ho Seo2, Dongryeoul Bae1,†, Ji-Hee Park3,4, Saeed Khan1, Kidon Sung1,*
1Division of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR 72079, USA
2KU Center for One Health, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea
3Joy Animal Hospital, Ansan 15388, Korea
4Veterinary Clinical Pathology, College of Veterinary Medicine, Chonbuk National University, Jeonju 54896, Korea
Correspondence to: Tel: +1-870-543-7527; Fax: +1-870-543-7307; E-mail: Kidon.Sung@fda.hhs.gov
Present address: Arkansas Regional Laboratory, Office of Regulatory, U.S. Food and Drug Administration, Jefferson, AR 72079, USA
Received: June 12, 2017; Revised: January 18, 2018; Accepted: February 10, 2018; Published online: May 31, 2018.
Abstract
Clostridium perfringens causes diarrhea and other diseases in animals and humans. We investigated the prevalence, toxin gene profiles, and antibiotic resistance of C. perfringens isolated from diarrheic dogs (DD) and non-diarrheic dogs (ND) in two animal hospitals in Seoul, Korea. Fecal samples were collected from clinically DD (n = 49) and ND (n = 34). C. perfringens was isolated from 31 of 49 DD (63.3%) and 21 of 34 ND dogs (61.8%). All C. perfringens strains were positive for the α toxin gene, but not for the β, ε, or ι toxin genes; therefore, all strains were identified as type A C. perfringens. All isolates were cpe-negative, whereas the β2 toxin gene was identified in 83.9% and 61.9% of isolates from DD and ND, respectively. Most isolates were susceptible to ampicillin (94%), chloramphenicol (92%), metronidazole (100%), moxifloxacin (96%), and imipenem (100%). However, 25.0% and 21.2% of isolates were resistant to tetracycline and clindamycin, respectively. Molecular subtyping of the isolated strains was performed by using pulsed-field gel electrophoresis. Fifty-two isolates were classified into 48 pulsotypes based on more than 90% similarity of banding patterns. No notable differences were observed among the isolates from DD and ND.
Keywords: Clostridium perfringens, bacterial toxins, dogs, drug resistance, pulsed-field gel electrophoresis


© 2018 The Korean Society of Veterinary Science.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.