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J Vet Sci 2018; 19(3): 375-383  https://doi.org/10.4142/jvs.2018.19.3.375
Marek’s disease vaccine activates chicken macrophages
Dan Wang1, Shuhong Sun1, Mohammad Heidari2,*
1College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an 271018, China
2Avian Disease and Oncology Laboratory, U.S. Department of Agriculture, Agriculture Research Service (USDA-ARS), East Lansing, MI 48823, USA
Correspondence to: Tel: +1-517-337-6830; Fax: +1-517-337-6776; E-mail: mohammad.heidari@ars.usda.gov
Received: October 10, 2017; Revised: January 5, 2018; Accepted: January 10, 2018; Published online: May 31, 2018.
Abstract
To provide insights into the role of innate immune responses in vaccine-mediated protection, we investigated the effect of Marek’s disease (MD) vaccine, CVI988/Rispens, on the expression patterns of selected genes associated with activation of macrophages in MD-resistant and MD-susceptible chicken lines. Upregulation of interferon γ, interleukin (IL)-1β, IL-8, and IL-12 at different days post-inoculation (dpi) revealed activation of macrophages in both chicken lines. A strong immune response was induced in cecal tonsils of the susceptible line at 5 dpi. The highest transcriptional activities were observed in spleen tissues of the resistant line at 3 dpi. No increase in the population of CD3+ T cells was observed in duodenum of vaccinated birds at 5 dpi indicating a lack of involvement of the adaptive immune system in the transcriptional profiling of the tested genes. There was, however, an increase in the number of macrophages in the duodenum of vaccinated birds. The CVI988/Rispens antigen was detected in the duodenum and cecal tonsils of the susceptible line at 5 dpi but not in the resistant line. This study sheds light on the role of macrophages in vaccine-mediated protection against MD and on the possible development of new recombinant vaccines with enhanced innate immune system activation properties.
Keywords: CVI988/Rispens, Marek’s disease, cecal tonsils, duodenum, macrophages


© 2018 The Korean Society of Veterinary Science.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.