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J Vet Sci 2018; 19(3): 416-425  https://doi.org/10.4142/jvs.2018.19.3.416
Effect of immunization routes and protective efficacy of Brucella antigens delivered via Salmonella vector vaccine
Jonathan Lalsiamthara, Gayeon Won, John Hwa Lee*
College of Veterinary Medicine, Chonbuk National University, Iksan 54596, Korea
Correspondence to: Tel: +82-63-850-0940; Fax: +82-63-850-0910; E-mail: johnhlee@jbnu.ac.kr
Received: October 24, 2017; Revised: December 4, 2017; Accepted: January 10, 2018; Published online: May 31, 2018.
An anti-Brucella vaccine candidate comprised of purified Brucella lipopolysaccharide (LPS) and a cocktail of four Salmonella Typhimurium (ST)-Brucella vectors was reported previously. Each vector constitutively expressed highly conserved Brucella antigens (rB), viz., lumazine synthase (BLS), proline racemase subunit A, outer membrane protein-19 (Omp19), and Cu-Zn superoxide dismutase (SOD). The present study determined a relative level of protection conferred by each single strain. Upon virulent challenge, the challenge strain was recovered most abundantly in non-immunized control mice, with the ST-Omp19-, ST-BLS-, LPS-, and ST-SOD-immunized mice showing much less burden. Indirect enzyme-linked immunosorbent assay-based assay also confirmed the induction of antigen-specific immunoglobulin G for each antigen delivered. In a route-wise comparison of the combined vaccine candidate, intraperitoneal (IP), intramuscular (IM), and subcutaneous immunizations revealed an indication of highly efficient routes of protection. Splenocytes of mice immunized via IM and IP routes showed significant relative expression of IL-17 upon antigenic pulsing. Taken together, each of the Brucella antigens delivered by ST successfully induced an antigen-specific immune response, and it was also evident that an individual antigen strain can confer a considerable degree of protection. More effective protection was observed when the candidate was inoculated via IP and IM routes.
Keywords: Salmonella delivery, brucellosis, protective efficacy, vaccination

© 2018 The Korean Society of Veterinary Science.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.