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J Vet Sci 2018; 19(3): 452-461  https://doi.org/10.4142/jvs.2018.19.3.452
Clinical assessment after human adipose stem cell transplantation into dogs
Seok Hee Lee1, Erif M.N. Setyawan1, Yoo Bin Choi1, Jeong Chan Ra2, Sung Keun Kang2, Byeong Chun Lee1, Geon A Kim1,*
1Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea
2Biostar Stem Cell Research Institute, R Bio Co., Ltd., Seoul 08506, Korea
Correspondence to: Tel: +82-2-880-1269; Fax: +82-2-873-1269; E-mail: bclee@snu.ac.kr
Received: October 10, 2017; Revised: December 15, 2017; Accepted: December 26, 2017; Published online: May 31, 2018.
Abstract
Adipose tissue-derived stem cell (ASCs) are an attractive source of stem cells with therapeutic applicability in various fields for regenerating damaged tissues because of their stemness characteristics. However, little has reported on evaluating adverse responses caused by human ASC therapy. Therefore, in the present study, a clinical assessment after human ASC transplantation into dogs was undertaken. A total of 12 healthy male dogs were selected and divided into four groups: saline infusion, saline bolus, ASC infusion, and ASC bolus groups. Physical assessment and blood analysis were performed following ASC transplantation, and the concentrations of angiogenic factors, and pro- and anti-inflammatory cytokines were measured by enzyme-linked immunosorbent assay (ELISA). There were no adverse vital sign responses among the dogs. Blood analyses revealed no remarkable complete blood count or serum chemistry results. ELISA results for angiogenic and anti-inflammatory factors including matrix metalloproteinase 9 (MMP9), vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), hepatocyte growth factor (HGF), and interleukin-10 (IL-10) were significantly higher in the two ASCs groups than in the controls. In conclusion, this study demonstrated that transplantation of human ASCs produced no adverse effects and could be used safely in dogs. In addition, human ASCs could be involved in modulating secretions of angiogenic factors including MMP9, VEGF, bFGF, and HGF and anti-inflammatory factor IL-10.
Keywords: adipose stem cells, angiogenic factors, anti-inflammatory factor, dogs, transplantation


© 2018 The Korean Society of Veterinary Science.

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