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Genetic diversity of bovine Mycobacterium avium subsp. paratuberculosis discriminated by IS1311 PCR-REA, MIRU-VNTR and MLSSR genotyping
Hong-Tae Park1, Hyun-Eui Park1, Woo Bin Park1, Suji Kim1, Young-Hoon Jung2, Han Sang Yoo1,3,*
1Department of Infectious Disease, College of Veterinary Medicine, Seoul National University, Seoul, Korea
2Department of Animal Resources Development, National Institute of Animal Science, Rural Development Administration, Cheonan, Korea
3Institute of Green Bio Science and Technology, Seoul National University, Pyeongchang, Korea
Correspondence to: Tel: +82-2-880-1263; Fax: +82-2-874-2738; E-mail: yoohs@snu.ac.kr
Received: April 17, 2018; Revised: June 1, 2018; Accepted: June 4, 2018; Published online: June 21, 2018.
The aim of this study was to describe the genetic diversity of Mycobacterium avium subsp. paratuberculosis (MAP) obtained from individual cows in Korea. Twelve MAP-positive fecal DNA samples and 19 MAP isolates were obtained from 10 cattle herds located in 5 provinces in Korea. In addition to this, 5 MAP isolates were obtained from the Czech Republic and Slovakia, and 3 were from Australia were used for typing to compare with the domestic isolates. The most prevalent strains in Korea were the “bison-type” genotype (23 out of the 31 fecal DNA/isolates) and distributed nationwide. Remaining MAP isolates (8) and all of the foreign isolates were identified as cattle-type. The bison-type strains which were discriminated only as INMV 68 in MIRU-VNTR. MLSSR typing differentiated bison-type strains into 3 different subtypes. Cattle-type was divided into 3 subtypes by MIRU-VNTR and 8 subtypes by MLSSR. The allelic diversities of MIRU-VNTR and MLSSR were calculated as 0.567 and 0.866, respectively. These results suggest that MIRU-VNTR typing method cannot provide sufficient description of epidemiological situation of MAP. Therefore, alternative method like MLSSR is needed for typing of MAP strains to understand the molecular epidemiology of MAP infections. Overall, this study is the first epidemiological survey report in Korea using MIRU-VNTR and MLSSR typing methods, which has provided basic data necessary to understand the situation of MAP infections in Korea.
Keywords: Mycobacterium avium subsp. Paratuberculosis, IS1311 PCR-REA, MIRU-VNTR, MLSSR, Korea

© 2018 The Korean Society of Veterinary Science.