• home
  • articles
  • authors
  • Reviewers
  • About the Journal
  • About the Journal
  • About the Journal
  • About the Journal
  • e-Submission

Indexed/Covered by

Vaccination of goats with a combination of Salmonella vector expressing Brucella antigens (BLS, PrpA, Omp19, SOD) confers a protection against Brucella abortus infection
Mwense Leya1,†, Won Kyong Kim1,†, Jeong Sang Cho1, Eun-Chae Yu2, Young-Jee Kim2, Yoonhwan Yeo2, Kwang-Soo Lyoo2, Myeon-Sik Yang1, Sang-Seop Han2, John Hwa Lee1, Dongseob Tark2, Jin Hur1, Bumseok Kim1,*
1College of Veterinary Medicine, and 2Korea Zoonosis Research institute, Chonbuk National University, Iksan, 54596, Korea
Correspondence to: Tel: +82-63-850-0953; Fax: +82-63-850-0980; E-mail: bskims@jbnu.ac.kr
The first two authors contributed equally to this work.
Received: April 18, 2018; Revised: June 2, 2018; Accepted: June 10, 2018; Published online: June 21, 2018.
Abstract
Salmonella is an intracellular pathogen exhibiting a cellular infection mechanism similar to that of Brucella, making it a suitable choice for an anti-Brucella immune boost system. This study explores the efficacy of a Salmonella Typhimurium delivery-based combination vaccine for heterologous Brucella antigens (rBs) including Brucella lumazine synthase (BLS), proline racemase subunit A (PrpA), outer-membrane protein 19 (Omp19), and Cu/Zn superoxide dismutase (SOD) targeting brucellosis in goats. We inoculated the attenuated Salmonella delivery-based vaccine combination at two different subcutaneous inoculations; 5 × 109 CFU/ml (group B) and 5 × 1010 CFU/ml (group C) and challenged it with virulent Brucella abortus at 6 weeks post-immunization. Serum IgG titers against individual antigens in Salmonella immunized goats (group C) were significantly increased compared to those of the non-immunized goats (group A) at 3 and 6 weeks after the vaccine injection. IFN-γ from PBMCs was significantly elevated in group B and C compared to group A upon antigenic stimulation. The immunized goats showed significantly higher protection as demonstrated by lower bacterial load in most of the tissues of the goats challenged with Brucella abortus. A relative real-time PCR technique revealed that the expression of Brucella antigens was decreased more in the spleen, kidney, and lungs of the immunized goats than in the non-immunized animals. Also, treatment with our combination vaccine ameliorated histopathological lesions induced by the Brucella infection. Overall, the Salmonella Typhimurium delivery-based combination vaccine was effective in delivering immunogenic Brucella proteins, making it a potential success for use in livestock.
Keywords: Brucellosis, Brucella vaccine, Salmonella delivery, Protective efficacy, Goat


© 2018 The Korean Society of Veterinary Science.