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J Vet Sci 2018; 19(4): 477-482  https://doi.org/10.4142/jvs.2018.19.4.477
Porcine intestinal lymphoid tissues synthesize estradiol
Chan Jin Park1,†, Heehyen Kim1,2,†, Jooyoung Jin1,2,†, Radwa Barakat1,3, Po-Ching Lin1, Jeong Moon Choi1, CheMyong Jay Ko1,*
1Department of Comparative Biosciences, College of Veterinary Medicine, University of Illinois, Urbana-Campaign, IL 61802, USA
2Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Korea
3Department of Toxicology, Faculty of Veterinary Medicine, Benha University, Benha 13518, Egypt
Correspondence to: Tel: +1-217-333-9362; E-mail: jayko@illinois.edu
The first three authors contributed equally to this work.
Received: November 29, 2017; Revised: February 5, 2018; Accepted: February 10, 2018; Published online: July 31, 2018.
Abstract
Estradiol (17β-estradiol) is synthesized primarily in the gonads of both sexes and regulates the development and function of reproductive organs. Recently, we reported that intestinal lymphocyte homeostasis is regulated by estradiol synthesized de novo in the endothelial cells of the high endothelial venules (HEVs) of mesenteric lymph nodes and Peyer’s patches in mice. This observation prompted us to hypothesize that HEVs of intestinal lymphoid tissues are sites of estradiol synthesis across species. In this study, we examined whether estradiol is synthesized in the intestinal lymphoid tissues of adolescent piglets. Comparisons of estradiol levels in blood and tissue showed that estradiol concentrations in mesenteric lymph nodes and Peyer’s patches were significantly higher than the level in serum. Reverse transcription polymerase chain reaction showed that porcine intestinal lymphoid tissues express mRNAs for steroidogenic enzymes (StAR, 17β-Hsd, 3β-Hsd, Cyp17a1, and Cyp19a1), and immunohistochemical results in ilial tissue showed expression of aromatase (CYP19) in Peyer’s patch-localized endothelial cells of HEVs. When mesenteric lymph node and Peyer’s patch tissues were cultured in vitro, they produced estradiol. Taken together, the results indicate that mesenteric lymph nodes and Peyer’s patches are sites of estradiol synthesis in adolescent piglets.
Keywords: aromatase, estradiol, intestines, lymph nodes, swine


© 2018 The Korean Society of Veterinary Science.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.