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J Vet Sci 2018; 19(4): 519-527  https://doi.org/10.4142/jvs.2018.19.4.519
An enhanced immunochromatographic strip test using colloidal gold nanoparticle-labeled dual-type N proteins for detection of antibodies to PRRS virus
Ji Eun Yu1, In-Ohk Ouh1, Hyeonjeong Kang1, Hye-young Lee2, Kwang-Myun Cheong2, In-Soo Cho1, Sang-Ho Cha1,*
1Viral Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea
2Research Institution, MEDIAN Diagnostics, Chuncheon 24399, Korea
Correspondence to: Tel: +82-54-912-0805; Fax: +82-54-912-0812; E-mail: virusmania@korea.kr
Received: October 30, 2017; Revised: February 21, 2018; Accepted: March 3, 2018; Published online: July 31, 2018.
Abstract
Porcine reproductive and respiratory syndrome (PRRS) is recognized as one of the most important infectious diseases causing serious economic loss in the swine industry worldwide. Due to its increasing genetic diversity, a rapid and accurate diagnosis is critical for PRRS control. The immunochromatographic strip test (ICST) is a rapid and convenient type of immunoassay. In this study, an on-site immunochromatographic assay-based diagnostic method was developed for detection of PRRS virus (PRRSV)-specific antibodies. The method utilized colloidal gold nanoparticle-labeled dual-type nucleocapsid proteins encoded by open reading frame 7. We evaluated 991 field samples from pig farms and 66 serum samples from experimentally PRRSV-inoculated pigs. Based on true PRRSV-specific antibody-positive or -negative sera determined by immunofluorescence assay and IgM enzyme-linked immunosorbent assay (ELISA), the specificity and sensitivity of the ICST were 97.5% and 91.1%, respectively, similar to those of a commercial ELISA (IDEXX PRRS X3 Ab). More importantly, the ICST was completed within 15 min and could detect the PRRSV-specific antibody at an earlier stage of infection (3-7 days) than that of ELISA (7+ days). The results demonstrate that the developed ICST has great potential as an on-farm diagnostic method, providing excellent diagnostic performance in a quick and convenient manner.
Keywords: immunochromatographic assay, on-farm detection, porcine reproductive and respiratory syndrome virus


© 2018 The Korean Society of Veterinary Science.

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