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J Vet Sci 2018; 19(4): 570-576  https://doi.org/10.4142/jvs.2018.19.4.570
Non-invasive quantification of hepatic fat content in healthy dogs by using proton magnetic resonance spectroscopy and dual gradient echo magnetic resonance imaging
Francesca Del Chicca1,2,*, Andrea Schwarz3, Dieter Meier4,5, Paula Grest6, Annette Liesegang7, Patrick R. Kircher1
1Clinic of Diagnostic Imaging, 3Section of Anesthesiology, Equine Department, and Institutes of 6Veterinary Pathology and 7Animal Nutrition, Vetsuisse Faculty, University of Zurich, 8057 Zurich, Switzerland
2Graduate School for Cellular and Biomedical Sciences, University of Bern, 3012 Bern, Switzerland
4Institute of Biomedical Engineering, University of Zurich, 8057 Zurich, Switzerland
5Swiss Federal Institute of Technology (ETH Zurich), 8092 Zurich, Switzerland
Correspondence to: Tel: +41-436358685; Fax: +41-446358940; E-mail: fdelchicca@vetclinics.uzh.ch
Received: October 25, 2017; Revised: February 19, 2018; Accepted: February 20, 2018; Published online: July 31, 2018.
Abstract
The objective of the present study was to describe two non-invasive methods for fat quantification in normal canine liver by using magnetic resonance imaging (MRI) and spectroscopy. Eleven adult beagle dogs were anesthetized and underwent magnetic resonance examination of the cranial abdomen by performing morphologic, modified Dixon (mDixon) dual gradient echo sequence, and proton magnetic resonance spectroscopy (1H MRS) imaging. In addition, ultrasonographic liver examination was performed, fine-needle liver aspirates and liver biopsies were obtained, and hepatic triglyceride content was assayed. Ultrasonographic, cytologic, and histologic examination results were unremarkable in all cases. The median hepatic fat fraction calculated was 2.1% (range, 1.3%–5.5%) using mDixon, 0.3% (range, 0.1%–1.0%) using 1H MRS, and 1.6% (range 1.0%–2.5%) based on triglyceride content. The hepatic fat fractions calculated using mDixon and 1H MRS imaging were highly correlated to that based on triglyceride content. A weak correlation between mDixon and 1H MRS imaging was detected. The results show that hepatic fat content can be estimated using non-invasive techniques (mDixon or 1H MRS) in healthy dogs. Further studies are warranted to evaluate the use of these techniques in dogs with varying hepatic fat content and different hepatic disorders.
Keywords: canine, hepatic triglyceride, liver, magnetic resonance imaging, proton magnetic resonance spectroscopy


© 2018 The Korean Society of Veterinary Science.

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