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Hexon and fiber gene changes in an attenuated fowl adenovirus isolate of Malaysia in chickens embryonated eggs and its infectivity in chickens.
MS Norfitriah1, M Hair-Bejo1,2,*, AR Omar1,2, I Aini1,2, MI Nurulfiza2,3
1Faculty of Veterinary Medicine, 2Institute of Bioscience, and 3Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor
Correspondence to: Tel: +603-86093888; Fax: +603-89471971; E-mail: mdhair@upm.edu.my
Received: February 23, 2018; Revised: July 29, 2018; Accepted: August 6, 2018; Published online: August 30, 2018.
Abstract
Fowl adenovirus (FAdV) is distributed worldwide and caused economic losses in poultry industry. The objectives of this study were to determine the hexon and fiber gene changes in an attenuated FAdV isolate of Malaysia in specific pathogen free (SPF) chicken embryonated eggs (CEE) and its infectivity in commercial broiler chickens. SPF CEE was inoculated with 0.1mL FAdV inoculum via chorioallantoic membrane (CAM) for 20 consecutive passages. The isolate at passage 20 (E20) with virus titer of 108.7TCID50/mL was inoculated (0.5mL) into day old commercial broiler chickens either via oral or intraperitoneal route. The study demonstrated that 100% embryonic mortality was recorded from passage 2 (E2) to E20 with delayed pattern at passage 17 onwards. The lesions were confined in the liver and CAM. Substitutions of amino acids in L1 loop of hexon at position 49 and 66, and in knob of fiber gene at position 318 and 322 were recorded in the E20 isolate. The isolate is belong to serotype 8b and non-pathogenic to broiler chickens, but able induce FAdV antibody titer. It appears that the molecular changes in L1 loop of hexon and knob of fiber genes are the marker for FAdV infectivity.
Keywords: Fowl adenovirus (FAdV); SPF chicken embryonated eggs; hexon and fiber genes; commercial broiler chickens; infectivity


© 2018 The Korean Society of Veterinary Science.