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Molecular prophage typing of Staphylococcus aureus isolates from bovine mastitis
Dae-Sung Ko1,2,†, Won-Jin Seong1,2,†, Danil Kim1, Eun-Kyung Kim1, Nam-Hyung Kim2, Chung-Young Lee2, Jae-Hong Kim2,3, Hyuk-Joon Kwon1,3,*
1Department of Farm Animal Medicine, 2Laboratory of Avian Diseases, and 3Research Institute for Veterinary Science, College of Veterinary Medicine and BK21 for Veterinary Science, Seoul National University, Seoul 08826, Korea
Correspondence to: Tel: +82-2-880-1226; Fax: +82-2-885-6614; E-mail: kwonhj01@snu.ac.kr
†The first two authors contributed equally to this work.
Received: April 20, 2018; Revised: August 14, 2018; Accepted: August 21, 2018; Published online: August 30, 2018.
Abstract
Staphylococcus aureus is one of the major pathogens causing bovine mastitis and foodborne disease associated with dairy products. To determine the genetic relationships between human and bovine or bovine isolates of S. aureus, various molecular methods have been used. Previously we developed an rpoB sequence typing (RSTing) method for molecular differentiation of S. aureus isolates and identification of RpoB-related antibiotic resistance. In this study, we performed spa typing and RSTing with 84 isolates from mastitic cows (22 farms, 72 cows, and 84 udders) and developed a molecular prophage typing (mPPTing) method for molecular epidemiological analysis of bovine mastitis. To compare the results, human isolates from patients (n = 14) and GenBank (n = 166) were used for real and in silico RSTing and mPPTing, respectively. According to the results, RST10-2 and RST4-1 were the most common RSTs in cows and humans, respectively, and most isolates from cows and humans clearly differed from each other. Antibiotic resistance-related RSTs were not detected in the cow isolates. A single dominant prophage type and gradual evolution through prophage acquisition were apparent in most of the tested farms. Thus, RSTing and mPPTing are informative and simple and economic methods for molecular epidemiological analysis of S. aureus infections
Keywords: Staphylococcus aureus; rpoB sequence typing; molecular prophage typing; bovine mastitis; molecular epidemiology


© 2018 The Korean Society of Veterinary Science.