• home
  • home
  • articles
  • authors
  • Reviewers
  • About the Journal
  • About the Journal
  • About the Journal
  • About the Journal
  • e-Submission

Indexed/Covered by

J Vet Sci 2018; 19(5): 620-626  https://doi.org/10.4142/jvs.2018.19.5.620
Detection of progressive and regressive phase and LINE-1 retrotransposon in transfected dogs with transmissible venereal tumor during chemotherapy
Sevil Atalay Vural1,*, Rifki Haziroglu1, Mehmet R. Vural2, Ibrahim M. Polat3, Arda S. Tunc1
Departments of 1Pathology and 2Obstetrics and Gynecology, Faculty of Veterinary Medicine, Ankara University, 06110 Ankara, Turkey
3Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Kirikkale University, 71450 Kirikkale, Turkey
Correspondence to: Tel: +90-312-3170315-4276; Fax: +90-312-3164472; E-mail: sevilvural@yahoo.com
Received: December 14, 2017; Revised: May 21, 2018; Accepted: June 25, 2018; Published online: September 30, 2018.
Canine transmissible venereal tumor (CTVT) is a tumor that commonly occurs in genital and extragenital sites of both genders. Long interspersed nuclear elements (LINE-1) retrotransposon has a pivotal role in allogenic transfection among uncontrolled dog populations. This study aimed to perform pathomorphological, immunohistochemical, and in situ polymerase chain reaction (PCR) evaluation of CTVT (n = 18) in transfected dogs during chemotherapy. Immunohistochemically, tumor phases were investigated by using specific markers (CD3, CD4, CD8, CD79, and transforming growth factor beta [TGF-β]), and investigated an amplified specific sequence of TVT LINE-1 retrotransposon by in situ PCR. Polyhedral-shaped neoplastic cells that had large, round, hypo/hyperchromatic nuclei and eosinophilic cytoplasm were detected. All marker results were positive, especially in the early weeks of recovery. CD4 and TGF-β markers were conspicuously positive at the initial stage. In situ PCR LINE-1 sequence was initially positive in only four cases. It is believed that the CD and TGF-β markers provide phase identification at tumor initiation and during chemotherapy. It is thought that presence of T and B lymphocytes, which have roles in cellular and humoral immunity, is needed so that regression of the tumor is possible.
Keywords: antineoplastic agent, immunohistochemistry, long interspersed nuclear element-1, venereal tumors

© 2018 The Korean Society of Veterinary Science.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.