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J Vet Sci 2018; 19(5): 643-652  https://doi.org/10.4142/jvs.2018.19.5.643
Vaccination of goats with a combination Salmonella vector expressing four Brucella antigens (BLS, PrpA, Omp19, and SOD) confers protection against Brucella abortus infection
Mwense Leya1,†, Won Kyong Kim1,†, Jeong Sang Cho1, Eun-Chae Yu2, Young-Jee Kim2, Yoonhwan Yeo2, Kwang-Soo Lyoo2, Myeon-Sik Yang1, Sang-Seop Han2, John Hwa Lee1, Dongseob Tark2, Jin Hur1, Bumseok Kim1,*
1College of Veterinary Medicine and 2Korea Zoonosis Research Institute, Chonbuk National University, Iksan 54596, Korea
Correspondence to: Tel: +82-63-850-0953; Fax: +82-63-850-0980; E-mail: bskims@jbnu.ac.kr
Received: April 18, 2018; Revised: June 2, 2018; Accepted: June 10, 2018; Published online: September 30, 2018.
Abstract
Salmonella is an intracellular pathogen with a cellular infection mechanism similar to that of Brucella, making it a suitable choice for use in an anti-Brucella immune boost system. This study explores the efficacy of a Salmonella Typhimurium delivery-based combination vaccine for four heterologous Brucella antigens (Brucella lumazine synthase, proline racemase subunit A, outer-membrane protein 19, and Cu/Zn superoxide dismutase) targeting brucellosis in goats. We inoculated the attenuated Salmonella delivery-based vaccine combination subcutaneously at two different inoculation levels; 5 × 109 colony-forming unit (CFU)/mL (Group B) and 5 × 1010 CFU/mL (Group C) and challenged the inoculations with virulent Brucella abortus at 6 weeks post-immunization. Serum immunoglobulin G titers against individual antigens in Salmonella immunized goats (Group C) were significantly higher than those of the non-immunized goats (Group A) at 3 and 6 weeks after vaccination. Upon antigenic stimulation, interferon-γ from peripheral blood mononuclear cells was significantly elevated in Groups B and C compared to that in Group A. The immunized goats had a significantly higher level of protection as demonstrated by the low bacterial loads in most tissues from the goats challenged with B. abortus. Relative real-time polymerase chain reaction results revealed that the expression of Brucella antigens was lower in spleen, kidney, and lung of immunized goats than of non-immunized animals. Also, treatment with our combination vaccine ameliorated histopathological lesions induced by the Brucella infection. Overall, the Salmonella Typhimurium delivery-based combination vaccine was effective in delivering immunogenic Brucella proteins, making it potentially useful in protecting livestock from brucellosis.
Keywords: Brucella vaccine, Salmonella vector, brucellosis, goats, protective efficacy


© 2018 The Korean Society of Veterinary Science.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.