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J Vet Sci 2018; 19(5): 667-675  https://doi.org/10.4142/jvs.2018.19.5.667
Effect of superoxide dismutase, catalase, and glutathione peroxidase supplementation in the extender on chilled semen of fertile and hypofertile dogs
Chiara Del Prete1,*, Francesca Ciani1, Simona Tafuri1, Maria Pia Pasolini1, Giovanni Della Valle1, Veronica Palumbo1, Lucia Abbondante2, Antonio Calamo1, Vincenza Barbato3, Roberto Gualtieri3, Riccardo Talevi3, Natascia Cocchia1
1Department of Veterinary Medicine and Animal Production, University of Naples Federico II, 80137 Naples, Italy
2Freelancer, 80137 Naples, Italy
3Department of Biology, University of Naples Federico II, 80126 Naples, Italy
Correspondence to: Tel: +39-81-2536017; Fax: +39-81-2536042; E-mail: chiara.delprete@unina.it
Received: August 17, 2017; Revised: March 23, 2018; Accepted: April 4, 2018; Published online: September 30, 2018.
This study investigated the correlation between oxidative stress status and key canine sperm parameters and the effect of addition of a superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) combination in egg yolk tris-citrate glucose (EYT-G) extender on semen during 10 days of storage at 4. Ten Boxer dogs were divided into two groups, fertile (F) and hypofertile (H), depending on pregnancy and live birth rate status in the previous year. Semen evaluation was performed on the day of collection (D0) and after 5 (D5) and 10 (D10) days of cooled storage. Sperm motility, kinetic parameters, and DNA integrity were assessed. A correlation between oxidative status and key semen parameters in both F and H groups was observed. Total and progressive motilities were significantly higher in the treated (SOD, CAT, and GPx addition) versus control groups at D10 in both F and H groups, and at D5 in the H group. DNA integrity was significantly higher in both treated groups (H and F) at D5 and D10. In conclusion, the addition of SOD, CAT, and GPx in the extender allows preservation of semen quality for up to 10 days of storage at 4 in both fertile and hypofertile dogs.
Keywords: antioxidants, canine sperm, fertility, oxidative stress, semen preservation 

© 2018 The Korean Society of Veterinary Science.

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