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Dapsone modulates lipopolysaccharide-activated bone marrow cells by inducing cell death and down-regulating tumor necrosis factor-alpha production
Min-Ji Kwon1, Hong-Gu Joo1,2,*
1College of Veterinary Medicine, 2Veterinary Medical Research Institute, Jeju National University, Jeju 63243, Republic of Korea
Correspondence to: Tel: +82-64-754-3379; Fax: +82-64-756-3354; E-mail: jooh@jejunu.ac.kr
Received: June 1, 2018; Revised: August 26, 2018; Accepted: September 3, 2018; Published online: October 8, 2018.
Abstract
Dapsone, an antibiotic, has been used to cure leprosy. Although it was reported that dapsone has anti-inflammatory activity in hosts. However, the anti-inflammatory mechanism of dapsone has not been elucidated enough. The present study investigated the anti-inflammatory effects of dapsone on bone marrow cells (BMs), especially upon the exposure of lipopolysaccharide (LPS). We treated BMs with LPS and dapsone. The treated cells were used for cellular activity assay, flow cytometry analysis, the cytokine production, and reactive oxygen species assay. LPS distinctly activated BMs with several characters including high cellular activity, the change of granulocytes, and the increase of tumor necrosis factor (TNF)-alpha production. Interestingly, dapsone modulated the inflammatory cells including granulocytes in LPS-treated BMs by inducing cell death. While the percentage of Gr-1 positive cells was 57% in control cells, LPS increased to 75% and LPS plus dapsone decreased to 64%. Furthermore, dapsone decreased the mitochondrial membrane potential of LPS-treated BMs. In spite of a low concentration (25 µg/mL), dapsone significantly decreased the production of TNF-alpha in LPS-treated BMs by 54%. This study confirmed that dapsone has anti-inflammatory effects on LPS-mediated inflammation by modulating the number and function of inflammatory cells, providing new and useful information for clinicians and researchers.
Keywords: Dapsone, Bone marrow cells, Lipopolysaccharide, Cell death, Granulocytes


© 2018 The Korean Society of Veterinary Science.